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Reproductive capacity of neo-tetraploid loaches produced using diploid spermatozoa from a natural tetraploid male

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タイトル: Reproductive capacity of neo-tetraploid loaches produced using diploid spermatozoa from a natural tetraploid male
著者: Fujimoto, Takafumi 著作を一覧する
Yasui, George Shigueki 著作を一覧する
Hayakawa, Mayumi 著作を一覧する
Sakao, Suzu 著作を一覧する
Yamaha, Etsuro 著作を一覧する
Arai, Katsutoshi 著作を一覧する
キーワード: polyploid
chromosome manipulation
hybrid
sterile
発行日: 2010年
出版者: Elsevier
誌名: Aquaculture
巻: 308
開始ページ: S133
終了ページ: S139
抄録: Tetraploid loaches have been discovered amongst specimens recovered from fish markets in Japan. These tetraploids can be used as a source of diploid gametes to assist the further expansion of ploidy manipulation. Here, we produced a first generation neo-tetraploid strain by fertilizing eggs from a normal diploid female with diploid spermatozoa from a natural tetraploid male, followed five minutes later by heat shock (42°C, 2 min duration) to inhibit release of the second polar body. Diploid spermatozoa from the neo-tetraploid males produced were then used to create androgenetic diploid progeny by fertilizing UV-irradiated eggs from a normal diploid female. Triploid progeny were produced by crossing a normal diploid female with a neo-tetraploid male. Tetraploid progeny were produced by cold shock (1°C, 40 min duration), beginning 5 min after fertilizing normal eggs with diploid spermatozoa from first generation neo-tetraploid males. Reproductive performance of second generation progeny was also examined. Androgenetic diploid males generated fertile haploid spermatozoa. Triploid males were sterile, but a triploid female laid fertile haploid eggs. Second generation neo-tetraploid males were considered sterile.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/44224
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 荒井 克俊

 

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