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Reproductive capacity of neo-tetraploid loaches produced using diploid spermatozoa from a natural tetraploid male

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Title: Reproductive capacity of neo-tetraploid loaches produced using diploid spermatozoa from a natural tetraploid male
Authors: Fujimoto, Takafumi Browse this author
Yasui, George Shigueki Browse this author
Hayakawa, Mayumi Browse this author
Sakao, Suzu Browse this author
Yamaha, Etsuro Browse this author
Arai, Katsutoshi Browse this author →KAKEN DB
Keywords: polyploid
chromosome manipulation
hybrid
sterile
Issue Date: 2010
Publisher: Elsevier
Journal Title: Aquaculture
Volume: 308
Start Page: S133
End Page: S139
Abstract: Tetraploid loaches have been discovered amongst specimens recovered from fish markets in Japan. These tetraploids can be used as a source of diploid gametes to assist the further expansion of ploidy manipulation. Here, we produced a first generation neo-tetraploid strain by fertilizing eggs from a normal diploid female with diploid spermatozoa from a natural tetraploid male, followed five minutes later by heat shock (42°C, 2 min duration) to inhibit release of the second polar body. Diploid spermatozoa from the neo-tetraploid males produced were then used to create androgenetic diploid progeny by fertilizing UV-irradiated eggs from a normal diploid female. Triploid progeny were produced by crossing a normal diploid female with a neo-tetraploid male. Tetraploid progeny were produced by cold shock (1°C, 40 min duration), beginning 5 min after fertilizing normal eggs with diploid spermatozoa from first generation neo-tetraploid males. Reproductive performance of second generation progeny was also examined. Androgenetic diploid males generated fertile haploid spermatozoa. Triploid males were sterile, but a triploid female laid fertile haploid eggs. Second generation neo-tetraploid males were considered sterile.
Type: article (author version)
URI: http://hdl.handle.net/2115/44224
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 荒井 克俊

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