|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Reproductive capacity of neo-tetraploid loaches produced using diploid spermatozoa from a natural tetraploid male
| Title: | Reproductive capacity of neo-tetraploid loaches produced using diploid spermatozoa from a natural tetraploid male |
| Authors: | Fujimoto, Takafumi Browse this author | | Yasui, George Shigueki Browse this author | | Hayakawa, Mayumi Browse this author | | Sakao, Suzu Browse this author | | Yamaha, Etsuro Browse this author | | Arai, Katsutoshi Browse this author →KAKEN DB |
| Keywords: | polyploid | | chromosome manipulation | | hybrid | | sterile |
| Issue Date: | 2010 |
| Publisher: | Elsevier |
| Journal Title: | Aquaculture |
| Volume: | 308 |
| Start Page: | S133 |
| End Page: | S139 |
| Publisher DOI: | 10.1016/j.aquaculture.2010.04.029 |
| Abstract: | Tetraploid loaches have been discovered amongst specimens recovered from fish markets in Japan. These tetraploids can be used as a source of diploid gametes to assist the further expansion of ploidy manipulation. Here, we produced a first generation neo-tetraploid strain by fertilizing eggs from a normal diploid female with diploid spermatozoa from a natural tetraploid male, followed five minutes later by heat shock (42°C, 2 min duration) to inhibit release of the second polar body. Diploid spermatozoa from the neo-tetraploid males produced were then used to create androgenetic diploid progeny by fertilizing UV-irradiated eggs from a normal diploid female. Triploid progeny were produced by crossing a normal diploid female with a neo-tetraploid male. Tetraploid progeny were produced by cold shock (1°C, 40 min duration), beginning 5 min after fertilizing normal eggs with diploid spermatozoa from first generation neo-tetraploid males. Reproductive performance of second generation progeny was also examined. Androgenetic diploid males generated fertile haploid spermatozoa. Triploid males were sterile, but a triploid female laid fertile haploid eggs. Second generation neo-tetraploid males were considered sterile. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/44224 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 荒井 克俊
|
