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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Restoration of the loach, Misgurnus anguillicaudatus, from cryopreserved diploid sperm and induced androgenesis
| Title: | Restoration of the loach, Misgurnus anguillicaudatus, from cryopreserved diploid sperm and induced androgenesis |
| Authors: | Yasui, George Shigueki Browse this author | | Fujimoto, Takafumi Browse this author | | Arai, Katsutoshi Browse this author →KAKEN DB |
| Keywords: | Cryopreservation | | sperm bank | | androgenesis | | loach | | Misgurnus anguillicaudatus | | teleost |
| Issue Date: | 2010 |
| Publisher: | ELSEVIER |
| Journal Title: | Aquaculture |
| Volume: | 308 |
| Start Page: | S140 |
| End Page: | S144 |
| Publisher DOI: | 10.1016/j.aquaculture.2010.05.041 |
| Abstract: | In the present study, we evaluated the feasibility of using cryopreserved diploid sperm as a repository genebank for the loach, Misgurnus anguillicaudatus, along with a rederivation strategy utilising induced-androgenesis. Firstly, we evaluated three types of media for egg inactivation: Hank's saline solution + 0.5% bovine serum albumin (BSA), Ringer's solution + 0.5% BSA, and masu salmon seminal plasma. Haploid and diploid sperm were taken from diploid and tetraploid loaches, respectively. Fresh and cryopreserved haploid or diploid sperm were then used to fertilize intact or UV-irradiated eggs from wild diploid females. The irradiation media evaluated here successfully maintained the egg quality over 2 hours. Fertilization and hatching rates of eggs fertilized with cryopreserved diploid sperm were 11.68 ± 6.74% and 7.14 ± 6.29% respectively, compared to 63.51 ± 10.68% and 45.19 ± 16.2% for intact eggs fertilized by fresh haploid sperm. All-male inheritance was confirmed by determination of larval morphology, ploidy status and microsatellite genotypes of putative androgenetic progeny. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/44225 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 荒井 克俊
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