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Biological activities of Bacteroides forsythus lipoproteins and their possible pathological roles in periodontal disease

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Title: Biological activities of Bacteroides forsythus lipoproteins and their possible pathological roles in periodontal disease
Authors: Hasebe, A. Browse this author
Yoshimura, A. Browse this author
Into, T. Browse this author
Kataoka, H. Browse this author
Tanaka, S. Browse this author
Arakawa, S. Browse this author
Ishikura, H. Browse this author
Golenbock, DT Browse this author
Sugaya, T. Browse this author
Tsuchida, N. Browse this author
Kawanami, M. Browse this author
Hara, Y. Browse this author
Shibata, K. Browse this author →KAKEN DB
Issue Date: Mar-2004
Publisher: American Society For Microbiology
Journal Title: Infection and immunity
Volume: 72
Issue: 3
Start Page: 1318
End Page: 1325
Publisher DOI: 10.1128/IAI.72.3.1318-1325.2004.
PMID: 14977934
Abstract: Bacteroides forsythus is a gram-negative, anaerobic, fusiform bacterium and is considered to be an etiological agent in periodontal disease. A lipoprotein fraction prepared from B. forsythus cells by Triton X-114 phase separation (BfLP) activated human gingival fibroblasts and a human monocytic cell line, THP-1, to induce interleukin-6 production and tumor necrosis factor alpha production. BfLP was found to be capable of inducing nuclear factor-kappaB translocation in human gingival fibroblasts and THP-1 cells. By using Chinese hamster ovary K1 cells transfected with Toll-like receptor genes together with a nuclear factor-kappaB-dependent CD25 reporter plasmid, it was found that signaling by BfLP was mediated by Toll-like receptor 2 but not by CD14 or Toll-like receptor 4. BfLP induced apoptotic cell death in human gingival fibroblasts, KB cells (an oral epithelial cell line), HL-60 cells (a human myeloid leukemia cell line), and THP-1 cells but not in MOLT4 cells (a T-cell leukemia cell line). Caspase-8, an initiator caspase in apoptosis, was found to be activated in these cells in response to BfLP stimulation. Thus, this study suggested that BfLP plays some etiological roles in oral infections, especially periodontal disease, by induction of cell activation or apoptosis.
Type: article (author version)
URI: http://hdl.handle.net/2115/44228
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 長谷部 晃

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