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Promotion of callus propagation by 5-aminolevulinic acid in a Laminaria japonica sporophyte

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Title: Promotion of callus propagation by 5-aminolevulinic acid in a Laminaria japonica sporophyte
Authors: Tabuchi, Katsuhiro Browse this author
Mizuta, Hiroyuki Browse this author →KAKEN DB
Yasui, Hajime Browse this author →KAKEN DB
Keywords: 5-aminolevulinic acid
Issue Date: Dec-2009
Publisher: Blackwell Publishing
Journal Title: Aquaculture Research
Volume: 41
Issue: 1
Start Page: 1
End Page: 10
Publisher DOI: 10.1111/j.1365-2109.2009.02294.x
Abstract: The effects of 5-aminolevulinic acid (ALA) on the induction and growth of callus-like cells in Laminaria japonica were investigated in explants obtained from basal, middle and apical portions along the sporophyte. ALA treatment promoted the induction of callus-like cells in explants obtained from all portions, and the induction rate was higher when a concentration of 50-500 mg L^[-1] of ALA was used. The promotion was especially remarkable in apical explants, and the induction was 10-14 times higher in the 100-500 mg L^[-1] range than that in the 0 mg L^[-1]. The cell division rate of callus-like cells showed the highest value in the explants cultured with 500 mg L^[-1] of ALA for 14 days. The promotion of the cell division rate by culturing with 500 mg L^[-1] ALA was also observed under white, blue and red lights. The callus-like cells, which were cultured in 500 mg L^[-1] of ALA for 2 months, had many clear chloroplasts. After 3 months, young thalli occurred. These results suggest that the ALA treatment is effective for stable propagation of callus-like cells in L. japonica.
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Type: article (author version)
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 水田 浩之

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