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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
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Promotion of callus propagation by 5-aminolevulinic acid in a Laminaria japonica sporophyte
| Title: | Promotion of callus propagation by 5-aminolevulinic acid in a Laminaria japonica sporophyte |
| Authors: | Tabuchi, Katsuhiro Browse this author | | Mizuta, Hiroyuki Browse this author →KAKEN DB | | Yasui, Hajime Browse this author →KAKEN DB |
| Keywords: | 5-aminolevulinic acid | | callus | | development | | Laminaria | | sporophyte |
| Issue Date: | Dec-2009 |
| Publisher: | Blackwell Publishing |
| Journal Title: | Aquaculture Research |
| Volume: | 41 |
| Issue: | 1 |
| Start Page: | 1 |
| End Page: | 10 |
| Publisher DOI: | 10.1111/j.1365-2109.2009.02294.x |
| Abstract: | The effects of 5-aminolevulinic acid (ALA) on the induction and growth of callus-like cells in Laminaria japonica were investigated in explants obtained from basal, middle and apical portions along the sporophyte. ALA treatment promoted the induction of callus-like cells in explants obtained from all portions, and the induction rate was higher when a concentration of 50-500 mg L^[-1] of ALA was used. The promotion was especially remarkable in apical explants, and the induction was 10-14 times higher in the 100-500 mg L^[-1] range than that in the 0 mg L^[-1]. The cell division rate of callus-like cells showed the highest value in the explants cultured with 500 mg L^[-1] of ALA for 14 days. The promotion of the cell division rate by culturing with 500 mg L^[-1] ALA was also observed under white, blue and red lights. The callus-like cells, which were cultured in 500 mg L^[-1] of ALA for 2 months, had many clear chloroplasts. After 3 months, young thalli occurred. These results suggest that the ALA treatment is effective for stable propagation of callus-like cells in L. japonica. |
| Rights: | The definitive version is available at www.blackwellsynergy.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/44307 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 水田 浩之
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