|
Hokkaido University Collection of Scholarly and Academic Papers >
Institute of Low Temperature Science >
Peer-reviewed Journal Articles, etc >
Blarina toxin, a mammalian lethal venom from the short-tailed shrew Blarina brevicauda : Isolation and characterization
| Title: | Blarina toxin, a mammalian lethal venom from the short-tailed shrew Blarina brevicauda : Isolation and characterization |
| Authors: | Kita, Masaki Browse this author | | Nakamura, Yasuo Browse this author | | Okumura, Yuushi Browse this author | | Ohdachi, Satoshi D. Browse this author →KAKEN DB | | Oba, Yuichi Browse this author | | Yoshikuni, Michiyasu Browse this author | | Kido, Hiroshi Browse this author | | Uemura, Daisuke Browse this author |
| Issue Date: | 18-May-2004 |
| Publisher: | The National Academy of Sciences |
| Journal Title: | Proceedings of the National Academy of Sciences |
| Volume: | 101 |
| Issue: | 20 |
| Start Page: | 7542 |
| End Page: | 7547 |
| Publisher DOI: | 10.1073/pnas.0402517101 |
| PMID: | 15136743 |
| Abstract: | Venomous mammals are rare, and their venoms have not been characterized. We have purified and characterized the blarina toxin (BLTX), a lethal mammalian venom with a tissue kallikrein-like activity from the submaxillary and sublingual glands of the short-tailed shrew Blarina brevicauda. Mice administered BLTX i.p. developed irregular respiration, paralysis, and convulsions before dying. Based on the amino acid sequence of purified protein, we cloned the BLTX cDNA. It consists of a prosequence and an active form of 253 aa with a typical catalytic triad of serine proteases, with a high identity with tissue kallikreins. BLTX is an N-linked microheterogeneous glycoprotein with a unique insertion of 10 residues, L106TFFYKTFLG115. BLTX converted kininogens to kinins, which may be one of the toxic pathogens, and had dilatory effects on the blood vessel walls. The acute toxicity and proteolytic activity of BLTX were strongly inhibited by aprotinin, a kallikrein inhibitor, suggesting that its toxicity is due to a kallikrein-like activity of the venom. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/44382 |
| Appears in Collections: | 低温科学研究所 (Institute of Low Temperature Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 大舘 智志
|
