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Ciliates rapidly enhance the frequency of conjugation between Escherichia coli strains through bacterial accumulation in vesicles

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Title: Ciliates rapidly enhance the frequency of conjugation between Escherichia coli strains through bacterial accumulation in vesicles
Authors: Matsuo, Junji Browse this author →KAKEN DB
Oguri, Satoshi Browse this author
Nakamura, Shinji Browse this author →KAKEN DB
Hanawa, Tomoko Browse this author
Fukumoto, Tatsuya Browse this author
Hayashi, Yasuhiro Browse this author →KAKEN DB
Kawaguchi, Kouhei Browse this author
Mizutani, Yoshihiko Browse this author
Yao, Takashi Browse this author
Akizawa, Kouzi Browse this author
Suzuki, Haruki Browse this author
Simizu, Chikara Browse this author
Matsuno, Kazuhiko Browse this author →KAKEN DB
Kamiya, Shigeru Browse this author
Yamaguchi, Hiroyuki Browse this author →KAKEN DB
Keywords: Protozoa
Escherichia coli
Issue Date: Oct-2010
Publisher: Elsevier Masson SAS
Journal Title: Research in Microbiology
Volume: 161
Issue: 8
Start Page: 711
End Page: 719
Publisher DOI: 10.1016/j.resmic.2010.07.004
PMID: 20691258
Abstract: The mechanism underlying bacterial conjugation through protozoa was investigated. Kanamycin-resistant Escherichia coli SM10λ+ carrying pRT733 with TnphoA was used as donor bacteria and introduced by conjugation into ciprofloxacin-resistant E. coli clinical isolate recipient bacteria. Equal amounts of donor and recipient bacteria were mixed together in the presence or absence of protozoa (ciliates, free-living amoebae, myxamoebae) in Page's amoeba saline for 24 h. Transconjugants were selected with Luria Broth agar containing kanamycin and ciprofloxacin. The frequency of conjugation was estimated as the number of transconjugants for each recipient. Conjugation frequency in the presence of ciliates was estimated to be approximately 10^[-6], but in the absence of ciliates, or in the presence of other protozoa, it was approximately 10^[-8]. Conjugation also occurred in culture of ciliates at least 2 h after incubation. Successful conjugation was confirmed by polymerase chain reaction. Addition of cycloheximide or latrunculin B resulted in suppression of conjugation. Heat killing the ciliates or bacteria had no effect on conjugation frequency. Co-localization of green fluorescent protein-expressing E. coli and PKH67-vital stained E. coli was observed in the same ciliate vesicles, suggesting that both donor and recipient bacteria had accumulated in the same vesicle. In this study, the conjugation frequency of bacteria was found to be significantly higher in vesicles purified from ciliates than those in culture suspension. We conclude that ciliates rapidly enhance the conjugation of E. coli strains through bacterial accumulation in vesicles.
Type: article (author version)
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山口 博之

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