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EGFP-Rhm51 foci enable the visualization and enumeration of DNA double-strand breaks in Magnaporthe oryzae

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Title: EGFP-Rhm51 foci enable the visualization and enumeration of DNA double-strand breaks in Magnaporthe oryzae
Authors: Ndindeng, Sali Atanga Browse this author
Miki, Shinsuke Browse this author
Abe, Ayumi Browse this author
Asano, Kozo Browse this author
Sone, Teruo Browse this author
Keywords: Appressorium
DNA double-strand breaks
Rice colonization
Hyphal growth
Magnaporthe oryzae
Rice blast disease
Issue Date: Dec-2010
Publisher: Springer Japan
Journal Title: Journal of General Plant Pathology
Volume: 76
Issue: 6
Start Page: 377
End Page: 381
Publisher DOI: 10.1007/s10327-010-0271-0
Abstract: In order to detect and enumerate DNA double-strand breaks (DSBs) during the life cycle of Magnaporthe oryzae, an expression vector for GFP-Rhm51 fusion protein was constructed and introduced into the strain Ina86-137. Rhm51-GFP foci were detected and the number of foci in mitomycin-C-treated mycelia was higher than in untreated samples, indicating that the foci visualized DSBs occurred during the life cycle. Rhm51-GFP foci were observed in all stages of the asexual life cycle including the invasive hyphae formed in an intact rice leaf sheath, demonstrating that M. oryzae suffers DSBs during vegetative and infective growth.
Rights: The final publication is available at
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 曾根 輝雄

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