EGFP-Rhm51 foci enable the visualization and enumeration of DNA double-strand breaks in Magnaporthe oryzae

Ndindeng, Sali Atanga; Miki, Shinsuke; Abe, Ayumi; Asano, Kozo; Sone, Teruo

doi:10.1007/s10327-010-0271-0


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EGFP-Rhm51 foci enable the visualization and enumeration of DNA double-strand breaks in Magnaporthe oryzae

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/44584

Title:	EGFP-Rhm51 foci enable the visualization and enumeration of DNA double-strand breaks in Magnaporthe oryzae
Authors:	Ndindeng, Sali Atanga Browse this author
	Miki, Shinsuke Browse this author
	Abe, Ayumi Browse this author
	Asano, Kozo Browse this author
	Sone, Teruo Browse this author
Keywords:	Appressorium
	DNA double-strand breaks
	Rice colonization
	Hyphal growth
	Magnaporthe oryzae
	Rice blast disease
Issue Date:	Dec-2010
Publisher:	Springer Japan
Journal Title:	Journal of General Plant Pathology
Volume:	76
Issue:	6
Start Page:	377
End Page:	381
Publisher DOI:	10.1007/s10327-010-0271-0
Abstract:	In order to detect and enumerate DNA double-strand breaks (DSBs) during the life cycle of Magnaporthe oryzae, an expression vector for GFP-Rhm51 fusion protein was constructed and introduced into the strain Ina86-137. Rhm51-GFP foci were detected and the number of foci in mitomycin-C-treated mycelia was higher than in untreated samples, indicating that the foci visualized DSBs occurred during the life cycle. Rhm51-GFP foci were observed in all stages of the asexual life cycle including the invasive hyphae formed in an intact rice leaf sheath, demonstrating that M. oryzae suffers DSBs during vegetative and infective growth.
Rights:	The final publication is available at www.springerlink.com
Type:	article (author version)
URI:	http://hdl.handle.net/2115/44584
Appears in Collections:	農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 曾根輝雄

OAI-PMH ( junii2 , jpcoar_1.0 )

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