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GC Separation of cis-Eicosenoic Acid Positional Isomers on an Ionic Liquid SLB-IL100 Stationary Phase
| Title: | GC Separation of cis-Eicosenoic Acid Positional Isomers on an Ionic Liquid SLB-IL100 Stationary Phase |
| Authors: | Ando, Yasuhiro Browse this author →KAKEN DB | | Sasaki, Tsubasa Browse this author |
| Keywords: | GC | | Eicosenoic acid | | Ionic liquid | | SLB-IL100 | | Fatty acid | | Fish oil | | Flathead flounder | | Herring | | Methyl ester |
| Issue Date: | Jun-2011 |
| Publisher: | Springer |
| Journal Title: | Journal of the American Oil Chemists' Society |
| Volume: | 88 |
| Issue: | 6 |
| Start Page: | 743 |
| End Page: | 748 |
| Publisher DOI: | 10.1007/s11746-010-1733-4 |
| Abstract: | Gas chromatography (GC) of cis-eicosenoic acid (20:1) positional isomers has been investigated on a capillary column of ionic liquid 1,9-di(3-vinyl-imidazolium)nonane bis(trifluoromethyl)sulfonylimidate stationary phase (SLB-IL100). A test mixture of isomeric 20:1 methyl esters was prepared from flathead flounder flesh lipids. On a 60-m column operated at 150-180 °C, six peaks appeared in the elution order of 20:ln-15 → 20:ln-13 → 20:ln-11 → 20:ln-9 → 20:ln-7 → 20:ln-5. These peaks were baseline resolved within 20 min at 180 °C. The 20:ln-13 and 20:ln-11 isomers, poorly resolved on conventional polar polysiloxane stationary phases, were completely separated from each other with separation factor α = 1.02 and peak resolution (Rs) >- 1.57. When equivalent chain length (ECL) values were compared between the SLB-IL100 and CP-Sil 88 (biscyanopropyl polysiloxane), those of 20:ln-15 and 20:ln-13 exceptionally tended to be lower on the SLB-IL100. The excellent separation of 20:1 isomers seems due to less retention of 20:ln-15 and 20:ln-13 on SLB-IL100 rather than simply due to its high polarity. Analysis of herring oil 20:1 revealed the occurrence of 20:ln-13 in the Pacific herring but not in the Atlantic herring. The ionic liquid stationary phase, SLB-IL100, is effective for analyzing 20:1 isomers occurring in fish and other natural oils. |
| Rights: | The original publication is available at springerlink.com. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/44587 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 安藤 靖浩
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