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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Rapid Preparation of Fluorescent 9-Anthrylmethyl Esters for Fatty Acid Analysis of Small Amount of Triacylglycerols
| Title: | Rapid Preparation of Fluorescent 9-Anthrylmethyl Esters for Fatty Acid Analysis of Small Amount of Triacylglycerols |
| Authors: | Ando, Yasuhiro Browse this author →KAKEN DB | | Haba, Yusuke Browse this author | | Soma, Kazutaka Browse this author | | Hiraoka, Yuko Browse this author | | Takatsu, Tetsuya Browse this author →KAKEN DB |
| Keywords: | 9-Anthrylmethyl ester | | Fatty acid | | Fish oil | | HPLC | | Transesterification | | Triacylglycerol |
| Issue Date: | Oct-2007 |
| Journal Title: | Lipids |
| Volume: | 42 |
| Issue: | 10 |
| Start Page: | 955 |
| End Page: | 960 |
| Publisher DOI: | 10.1007/s11745-007-3100-2 |
| PMID: | 17694342 |
| Abstract: | This paper proposes a one-step method for preparation of fluorescent 9-anthrylmethyl esters from triacylglycerols (TAG) ranging in amount from 0.1 to 5 μg. It involves base-catalyzed transesterification using potassium 9-anthracenemethoxide, prepared by proton exchange between 9-anthracenemethanol and potassium tert-butoxide. Transesterification for 10 min at room temperature gave the fatty acid 9-anthrylmethyl esters in nearly maximal yields (82–85%). The products could be analyzed by reversed-phase HPLC without purification. Excellent linear relationships were observed for standard curves of 10–250 pmol of TAG standards (16:0, 19:0, 18:2 and 22:6), and differences in the slopes were less than 5% among the standards. Almost consistent compositions of the esters were observed for the products formed from 0.5 to 5 μg or less of fish oils TAG, and they were similar to those obtained by HPLC of ordinary multi-step synthesis products and by GLC of methyl esters. The present method is a great improvement of derivatization time, and is powerful for fatty acid analysis of small amounts of natural TAG. |
| Rights: | The original publication is available at www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/44613 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 安藤 靖浩
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