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C-type lectins do not act as functional receptors for filovirus entry into cells
| Title: | C-type lectins do not act as functional receptors for filovirus entry into cells |
| Authors: | Matsuno, Keita Browse this author | | Nakayama, Eri Browse this author | | Noyori, Osamu Browse this author | | Marzi, Andrea Browse this author | | Ebihara, Hideki Browse this author | | Irimura, Tatsuro Browse this author →KAKEN DB | | Feldmann, Heinz Browse this author | | Takada, Ayato Browse this author →KAKEN DB |
| Keywords: | Filovirus | | Virus entry | | C-type lectin |
| Issue Date: | 3-Dec-2010 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 403 |
| Issue: | 1 |
| Start Page: | 144 |
| End Page: | 148 |
| Publisher DOI: | 10.1016/j.bbrc.2010.10.136 |
| Abstract: | Cellular C-type lectins have been reported to facilitate filovirus infection by binding to glycans on filovirus glycoprotein (GP). However, it is not clearly known whether interaction between C-type lectins and GP mediates all the steps of virus entry (i.e., attachment, internalization, and membrane fusion). In this study, we generated vesicular stomatitis viruses pseudotyped with mutant GPs that have impaired structures of the putative receptor binding regions and thus reduced ability to infect the monkey kidney cells that are routinely used for virus propagation. We found that infectivities of viruses with the mutant GPs dropped in C-type lectin-expressing cells, parallel with those in the monkey kidney cells, whereas binding activities of these GPs to the C-type lectins were not correlated with the infectivities. These results suggest that C-type lectin-mediated entry of filoviruses requires other cellular molecule(s) that may be involved in virion internalization or membrane fusion. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/44634 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 高田 礼人
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