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Differential effects of interleukin-12 and interleukin-15 on expansion of NK cell receptor-expressing CD8+ T cells
| Title: | Differential effects of interleukin-12 and interleukin-15 on expansion of NK cell receptor-expressing CD8+ T cells |
| Authors: | Sugita, Junichi Browse this author | | Tanaka, Junji Browse this author | | Yasumoto, Atsushi Browse this author | | Shiratori, Souichi Browse this author | | Wakasa, Kentaro Browse this author | | Kikuchi, Misato Browse this author | | Shigematsu, Akio Browse this author | | Kondo, Takeshi Browse this author | | Asaka, Masahiro Browse this author | | Imamura, Masahiro Browse this author |
| Keywords: | CD94 | | NKG2A | | NKG2D | | Cytolytic activity |
| Issue Date: | Feb-2010 |
| Publisher: | Springer Berlin / Heidelberg |
| Journal Title: | Annals of Hematology |
| Volume: | 89 |
| Issue: | 2 |
| Start Page: | 115 |
| End Page: | 120 |
| Publisher DOI: | 10.1007/s00277-009-0780-0 |
| PMID: | 19578849 |
| Abstract: | The cytolytic activity of cells expressing natural killer cell receptors (NKRs) depends on the balance between stimulatory and inhibitory signals. We investigated both inhibitory NK receptor (CD94/NKG2A) expression and stimulatory NKR (NKG2D) expression on T cells after stimulation with cytokines (IL-12 or IL-15). Cytolytic NKR-expressing CD8+ T cells were expanded from normal adult peripheral blood mononuclear cells using anti-CD3 monoclonal antibody and cytokines (IL-12 or IL-15). The proportion and absolute number of CD94/NKG2A-expressing T cells expanded by IL-12 were significantly larger than those of the cells expanded by IL-15. On the other hand, the proportion and absolute number of NKG2D-expressing T cells expanded by IL-15 were significantly larger than those of the cells expanded by IL-12. The proportions of NKG2D and intracellular granzyme A expression in CD94-expressing cells were much more increased in PBMCs cultured with IL-15 than those of cells cultured with IL-12. A real-time polymerase chain reaction assay showed that there was a 1.68-fold increase in NKG2D mRNA expression level and a 1.37-fold increase in DAP10 mRNA expression level in CD94-expressing cells expanded by IL-15 compared with those of the cells expanded by IL-12. The cytolytic activity levels of purified CD94-expressing cells from 8-day culture with IL-15 tested against 51Cr-labeled K562 cells by standard 4-h 51Cr release assays without prior sensitization were much higher than those of cells from 8-day culture with IL-12. IL-15 appears to be able to enhance the cytolytic activity of CD94/NKG2A-expressing cells through induction of NKG2D and intracellular granzyme expression much more efficiently than does IL-12. |
| Rights: | The original publication is available at www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/44772 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 杉田 純一
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