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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Role of intestinal Bifidobacterium pseudolongum in dietary fructo-oligosaccharide inhibition of 2,4-dinitrofluorobenzene-induced contact hypersensitivity in mice
| Title: | Role of intestinal Bifidobacterium pseudolongum in dietary fructo-oligosaccharide inhibition of 2,4-dinitrofluorobenzene-induced contact hypersensitivity in mice |
| Authors: | Sasajima, Naho Browse this author | | Ogasawara, Toru Browse this author | | Takemura, Naoki Browse this author | | Fujiwara, Reiko Browse this author | | Watanabe, Jun Browse this author | | Sonoyama, Kei Browse this author |
| Keywords: | Prebiotics | | Bifidobacteria | | Contact hypersensitivity | | Mice |
| Issue Date: | Feb-2010 |
| Publisher: | Cambridge University Press |
| Journal Title: | British Journal of Nutrition |
| Volume: | 103 |
| Issue: | 4 |
| Start Page: | 539 |
| End Page: | 548 |
| Publisher DOI: | 10.1017/S0007114509992145 |
| PMID: | 20003567 |
| Abstract: | Strategies to manipulate the gut microbiota have been explored for preventing allergy development. We previously showed that dietary supplementation with fructo-oligosaccharide (FOS) reduced 2, 4-dinitrofluorobenzene (DNFB)-induced contact hypersensitivity (CHS) in BALB/c mice. Because the CHS response was negatively correlated with the number of faecal bifidobacteria, particularly Bifidobacterium pseudolongum, the present Study aimed to examine whether oral administration of B. pseudolongum affects CHS response. Viable B. pseudolongum was successfully isolated from mouse faeces. Female BALB/c mice were fed a synthetic diet with or without FOS supplementation, and B. pseudolongum (2 X 10^[7] cells) was administered daily throughout the experimental period. Two weeks after starting the test diets, mice received DNFB on the ear auricle twice at 7-d intervals. Conventional cultivation and molecular biological analyses based on 16S rRNA gene sequences showed that administration of FOS and B. pseudolongum resulted in higher excretion of viable bifidobacteria, mainly B. psendolongum. Although dietary FOS reduced the CHS response as demonstrated by ear swelling, B. pseudolongum administration resulted in a reduction in the initial phase only of the CHS response. B. pseudolongum administration increased hapten-specific IgGI, while dietary FOS decreased IgG2a in sera. Administration of FOS and B. pseudolongum decreased interferon-γ production and increased IL-10 production in cervical lymph node cells restimulated with hapten in vitro. We conclude that B. pseudolongum proliferation in the intestinal tract is partially responsible for the reduction in DNFB-induced CHS response by dietary Supplementation with FOS in mice, which may be mediated by the modulation of antigen-induced cytokine production. |
| Rights: | © The Authors 2009 |
| Relation: | http://journals.cambridge.org/action/displayJournal?jid=BJN |
| Type: | article |
| URI: | http://hdl.handle.net/2115/44775 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 園山 慶
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