HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >

Maternal consumption of fructo-oligosaccharide diminishes the severity of skin inflammation in offspring of NC/Nga mice

Files in This Item:
BJN103_530-538.pdf408.33 kBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/44776

Title: Maternal consumption of fructo-oligosaccharide diminishes the severity of skin inflammation in offspring of NC/Nga mice
Authors: Fujiwara, Reiko Browse this author
Takemura, Naoki Browse this author
Watanabe, Jun Browse this author
Sonoyama, Kei Browse this author
Keywords: Fructo-oligosaccharide
Prebiotics
Atopic dermatitis
Gut microbiota
NC/Nga mice
Issue Date: Feb-2010
Publisher: Cambridge University Press
Journal Title: British Journal of Nutrition
Volume: 103
Issue: 4
Start Page: 530
End Page: 538
Publisher DOI: 10.1017/S000711450999198X
PMID: 19857365
Abstract: Strategies to manipulate the gut microbiota in infancy have been considered to prevent the development of allergic diseases later in life. We aimed to elucidate the effects of maternal dietary supplementation with a prebiotic oligosaccharide on gut microbiota and spontaneously developing atopic dermatitis-like skin lesions in the offspring of NC/Nga mice. Female NC/Nga mice were fed diets either with or without fructo-oligosaccharide supplementation during pregnancy and lactation. After weaning, offspring were fed the diets supplemented with or without fructo-oligosaccharide for 11 weeks in an air-uncontrolled conventional room. Changes in gut microbiota were assessed by denaturing gradient gel electrophoresis of the PCR-amplified 16S rRNA gene. Skin lesions were evaluated by a clinical score and scratching behaviour. Serum antibody levels were measured by ELISA, and expression levels of cytokines and chemokines in lesional tissue were evaluated by quantitative RT-PCR. Maternal supplementation with fructo-oligosaccharide modulated the gut microbiota in sucklings. Although maternal supplementation with fructo-oligosaccharide suppressed the increase in clinical skin severity score and scratching behaviour in offspring, dietary fructo-oligosaccharide after weaning was less effective. The diminution of skin lesions was accompanied by lower serum concentrations of total IgGl and lower expression levels of TNF-α in the lesional tissue. These data suggest that maternal consumption of fructo-oligosaccharide diminishes the severity of atopic dermatitis-like skin lesions in the offspring of NC/Nga mice.
Rights: © The Authors 2009
Relation: http://journals.cambridge.org/action/displayJournal?jid=BJN
Type: article
URI: http://hdl.handle.net/2115/44776
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 園山 慶

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

 - Hokkaido University