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Maternal consumption of fructo-oligosaccharide diminishes the severity of skin inflammation in offspring of NC/Nga mice
| Title: | Maternal consumption of fructo-oligosaccharide diminishes the severity of skin inflammation in offspring of NC/Nga mice |
| Authors: | Fujiwara, Reiko Browse this author | | Takemura, Naoki Browse this author | | Watanabe, Jun Browse this author | | Sonoyama, Kei Browse this author |
| Keywords: | Fructo-oligosaccharide | | Prebiotics | | Atopic dermatitis | | Gut microbiota | | NC/Nga mice |
| Issue Date: | Feb-2010 |
| Publisher: | Cambridge University Press |
| Journal Title: | British Journal of Nutrition |
| Volume: | 103 |
| Issue: | 4 |
| Start Page: | 530 |
| End Page: | 538 |
| Publisher DOI: | 10.1017/S000711450999198X |
| PMID: | 19857365 |
| Abstract: | Strategies to manipulate the gut microbiota in infancy have been considered to prevent the development of allergic diseases later in life. We aimed to elucidate the effects of maternal dietary supplementation with a prebiotic oligosaccharide on gut microbiota and spontaneously developing atopic dermatitis-like skin lesions in the offspring of NC/Nga mice. Female NC/Nga mice were fed diets either with or without fructo-oligosaccharide supplementation during pregnancy and lactation. After weaning, offspring were fed the diets supplemented with or without fructo-oligosaccharide for 11 weeks in an air-uncontrolled conventional room. Changes in gut microbiota were assessed by denaturing gradient gel electrophoresis of the PCR-amplified 16S rRNA gene. Skin lesions were evaluated by a clinical score and scratching behaviour. Serum antibody levels were measured by ELISA, and expression levels of cytokines and chemokines in lesional tissue were evaluated by quantitative RT-PCR. Maternal supplementation with fructo-oligosaccharide modulated the gut microbiota in sucklings. Although maternal supplementation with fructo-oligosaccharide suppressed the increase in clinical skin severity score and scratching behaviour in offspring, dietary fructo-oligosaccharide after weaning was less effective. The diminution of skin lesions was accompanied by lower serum concentrations of total IgGl and lower expression levels of TNF-α in the lesional tissue. These data suggest that maternal consumption of fructo-oligosaccharide diminishes the severity of atopic dermatitis-like skin lesions in the offspring of NC/Nga mice. |
| Rights: | © The Authors 2009 |
| Relation: | http://journals.cambridge.org/action/displayJournal?jid=BJN |
| Type: | article |
| URI: | http://hdl.handle.net/2115/44776 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 園山 慶
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