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Involvement of AtNAP1 in the regulation of chlorophyll degradation in Arabidopsis thaliana

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タイトル: Involvement of AtNAP1 in the regulation of chlorophyll degradation in Arabidopsis thaliana
著者: Nagane, Tomohiro 著作を一覧する
Tanaka, Ayumi 著作を一覧する
Tanaka, Ryouichi 著作を一覧する
キーワード: Arabidopsis thaliana
Chlorophyll metabolism
発行日: 2010年 3月
出版者: Springer Berlin / Heidelberg
誌名: Planta
巻: 231
号: 4
開始ページ: 939
終了ページ: 949
出版社 DOI: 10.1007/s00425-010-1099-8
抄録: In plants, chlorophyll is actively synthesized from glutamate in the developmental phase and degraded into non-fluorescent chlorophyll catabolites during senescence. The chlorophyll metabolism must be strictly regulated because chlorophylls and their intermediate molecules generate reactive oxygen species. Many mechanisms have been proposed for the regulation of chlorophyll synthesis including gene expression, protein stability, and feedback inhibition. However, information on the regulation of chlorophyll degradation is limited. The conversion of chlorophyll b to chlorophyll a is the first step of chlorophyll degradation. In order to understand the regulatory mechanism of this reaction, we isolated a mutant which accumulates 7-hydroxymethyl chlorophyll a (HMChl), an intermediate molecule of chlorophyll b to chlorophyll a conversion, and designated the mutant hmc1. In addition to HMChl, hmc1 accumulated pheophorbide a, a chlorophyll degradation product, when chlorophyll degradation was induced by dark incubation. These results indicate that the activities of HMChl reductase (HAR) and pheophorbide a oxygenase (PaO) are simultaneously down-regulated in this mutant. We identified a mutation in the AtNAP1 gene, which encodes a subunit of the complex for iron-sulfur cluster formation. HAR and PaO use ferredoxin as a reducing power and PaO has an iron-sulfur center; however, there were no distinct differences in the protein levels of ferredoxin and PaO between wild type and hmc1. The concerted regulation of chlorophyll degradation is discussed in relation to the function of AtNAP1.
Rights: The original publication is available at
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 田中 亮一


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