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Involvement of AtNAP1 in the regulation of chlorophyll degradation in Arabidopsis thaliana

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Title: Involvement of AtNAP1 in the regulation of chlorophyll degradation in Arabidopsis thaliana
Authors: Nagane, Tomohiro Browse this author
Tanaka, Ayumi Browse this author
Tanaka, Ryouichi Browse this author
Keywords: Arabidopsis thaliana
Chlorophyll metabolism
Issue Date: Mar-2010
Publisher: Springer Berlin / Heidelberg
Journal Title: Planta
Volume: 231
Issue: 4
Start Page: 939
End Page: 949
Publisher DOI: 10.1007/s00425-010-1099-8
PMID: 20087600
Abstract: In plants, chlorophyll is actively synthesized from glutamate in the developmental phase and degraded into non-fluorescent chlorophyll catabolites during senescence. The chlorophyll metabolism must be strictly regulated because chlorophylls and their intermediate molecules generate reactive oxygen species. Many mechanisms have been proposed for the regulation of chlorophyll synthesis including gene expression, protein stability, and feedback inhibition. However, information on the regulation of chlorophyll degradation is limited. The conversion of chlorophyll b to chlorophyll a is the first step of chlorophyll degradation. In order to understand the regulatory mechanism of this reaction, we isolated a mutant which accumulates 7-hydroxymethyl chlorophyll a (HMChl), an intermediate molecule of chlorophyll b to chlorophyll a conversion, and designated the mutant hmc1. In addition to HMChl, hmc1 accumulated pheophorbide a, a chlorophyll degradation product, when chlorophyll degradation was induced by dark incubation. These results indicate that the activities of HMChl reductase (HAR) and pheophorbide a oxygenase (PaO) are simultaneously down-regulated in this mutant. We identified a mutation in the AtNAP1 gene, which encodes a subunit of the complex for iron-sulfur cluster formation. HAR and PaO use ferredoxin as a reducing power and PaO has an iron-sulfur center; however, there were no distinct differences in the protein levels of ferredoxin and PaO between wild type and hmc1. The concerted regulation of chlorophyll degradation is discussed in relation to the function of AtNAP1.
Rights: The original publication is available at
Type: article (author version)
Appears in Collections:低温科学研究所 (Institute of Low Temperature Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 田中 亮一

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