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Spontaneous network activity visualized by ultra-sensitive Ca2+ indicators, yellow Cameleon-Nano

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/44903

Title: Spontaneous network activity visualized by ultra-sensitive Ca2+ indicators, yellow Cameleon-Nano
Authors: Horikawa, Kazuki Browse this author
Yamada, Yoshiyuki Browse this author
Matsuda, Tomoki Browse this author
Kobayashi, Kentarou Browse this author
Hashimoto, Mitsuhiro Browse this author
Matsu-ura, Toru Browse this author
Miyawaki, Atsushi Browse this author
Michikawa, Takayuki Browse this author
Mikoshiba, Katsuhiko Browse this author
Nagai, Takeharu Browse this author
Issue Date: Sep-2010
Publisher: Nature Publishing Group
Journal Title: Nature Methods
Volume: 7
Issue: 9
Start Page: 729
End Page: 732
Publisher DOI: 10.1038/nmeth.1488
PMID: 20693999
Abstract: We report a new set of ultra-sensitive Ca2+ indicators, yellow cameleon-Nano (YC-Nano), developed by engineering the Ca2+-sensing domain of a genetically encoded Ca2+ indicator, YC2.60 or YC3.60. Their high Ca2+ affinities (Kd = 15-140 nM) and large signal change (1,450%) enabled detection of subtle Ca2+ transients associated with intercellular signaling dynamics and neuronal activity, even in 100,000-cell networks. These indicators will be useful for studying information processing in living multi-cellular networks.
Type: article (author version)
URI: http://hdl.handle.net/2115/44903
Appears in Collections:電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 永井 健治

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