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Spontaneous network activity visualized by ultra-sensitive Ca2+ indicators, yellow Cameleon-Nano
Title: | Spontaneous network activity visualized by ultra-sensitive Ca2+ indicators, yellow Cameleon-Nano |
Authors: | Horikawa, Kazuki Browse this author | Yamada, Yoshiyuki Browse this author | Matsuda, Tomoki Browse this author | Kobayashi, Kentarou Browse this author | Hashimoto, Mitsuhiro Browse this author | Matsu-ura, Toru Browse this author | Miyawaki, Atsushi Browse this author | Michikawa, Takayuki Browse this author | Mikoshiba, Katsuhiko Browse this author | Nagai, Takeharu Browse this author |
Issue Date: | Sep-2010 |
Publisher: | Nature Publishing Group |
Journal Title: | Nature Methods |
Volume: | 7 |
Issue: | 9 |
Start Page: | 729 |
End Page: | 732 |
Publisher DOI: | 10.1038/nmeth.1488 |
PMID: | 20693999 |
Abstract: | We report a new set of ultra-sensitive Ca2+ indicators, yellow cameleon-Nano (YC-Nano), developed by engineering the Ca2+-sensing domain of a genetically encoded Ca2+ indicator, YC2.60 or YC3.60. Their high Ca2+ affinities (Kd = 15-140 nM) and large signal change (1,450%) enabled detection of subtle Ca2+ transients associated with intercellular signaling dynamics and neuronal activity, even in 100,000-cell networks. These indicators will be useful for studying information processing in living multi-cellular networks. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/44903 |
Appears in Collections: | 電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 永井 健治
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