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In vivo Evaluation of a Novel Chitosan/HAp Composite Biomaterial as a Carrier of rhBMP-2

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Title: In vivo Evaluation of a Novel Chitosan/HAp Composite Biomaterial as a Carrier of rhBMP-2
Authors: Kashiwazaki, Haruhiko Browse this author
Yamaguchi, Keisuke Browse this author
Harada, Naoki Browse this author
Akazawa, Toshiyuki Browse this author
Murata, Masaru Browse this author
Iizuka, Tadashi Browse this author
Ikoma, Toshiyuki Browse this author
Tanaka, Junzo Browse this author
Inoue, Nobuo Browse this author
Keywords: Chitosan
Hydroxyapatite (HAp)
Osteoinduction
Bioabsorption
Recombinant human bone morphogenetic protein-2 (rhBMP-2)
Issue Date: Dec-2010
Publisher: 硬組織再生生物学会
Journal Title: Journal of Hard Tissue Biology
Volume: 19
Issue: 3
Start Page: 181
End Page: 186
Publisher DOI: 10.2485/jhtb.19.181
Abstract: We developed a porous chitosan/hydroxyapatite (HAp) composite, in which the HAp nanocrystals align along the chitosan molecules, and examined the biocompatibility, osteoinductive activity, and the ability to act as a carrier of recombinant human bone morphogenetic protein-2 (rhBMP-2) of this novel biomaterial. The composite was subcutaneously implanted into the backs of 11-week-old SD rats, with or without rhBMP-2 (5μg). At 2 and 8 weeks after the implantation, the composite was explanted for morphohistological evaluation. In the presence of rhBMP-2, ectopic bone formation was found at 2 weeks and maturation of the newly formed bone around the composite at 8 weeks. Chitosan/HAp composite alone caused little inflammation, and new blood vessel growth and multinucleated giant cells were found around the composite, accompanied with roughening of the surface due to degradation at 2 weeks; however, neither cartilage nor bone formation was found around the composite. With rhBMP-2, the bioabsorption of the composite was accelerated as the rhBMP-2-induced bone matured. Histomorphometrical analysis showed that the mean value of the composite areas with rhBMP-2 was significantly smaller than that without rhBMT-2 at 2 and 8 weeks after the implantation. These results suggested that the novel chitosan/HAp composite was an effective bioabsorbable material as a carrier of rhBMP-2.
Type: article
URI: http://hdl.handle.net/2115/44925
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 柏崎 晴彦

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