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In vivo Evaluation of a Novel Chitosan/HAp Composite Biomaterial as a Carrier of rhBMP-2
| Title: | In vivo Evaluation of a Novel Chitosan/HAp Composite Biomaterial as a Carrier of rhBMP-2 |
| Authors: | Kashiwazaki, Haruhiko Browse this author | | Yamaguchi, Keisuke Browse this author | | Harada, Naoki Browse this author | | Akazawa, Toshiyuki Browse this author | | Murata, Masaru Browse this author | | Iizuka, Tadashi Browse this author | | Ikoma, Toshiyuki Browse this author | | Tanaka, Junzo Browse this author | | Inoue, Nobuo Browse this author |
| Keywords: | Chitosan | | Hydroxyapatite (HAp) | | Osteoinduction | | Bioabsorption | | Recombinant human bone morphogenetic protein-2 (rhBMP-2) |
| Issue Date: | Dec-2010 |
| Publisher: | 硬組織再生生物学会 |
| Journal Title: | Journal of Hard Tissue Biology |
| Volume: | 19 |
| Issue: | 3 |
| Start Page: | 181 |
| End Page: | 186 |
| Publisher DOI: | 10.2485/jhtb.19.181 |
| Abstract: | We developed a porous chitosan/hydroxyapatite (HAp) composite, in which the HAp nanocrystals align along the chitosan molecules, and examined the biocompatibility, osteoinductive activity, and the ability to act as a carrier of recombinant human bone morphogenetic protein-2 (rhBMP-2) of this novel biomaterial. The composite was subcutaneously implanted into the backs of 11-week-old SD rats, with or without rhBMP-2 (5μg). At 2 and 8 weeks after the implantation, the composite was explanted for morphohistological evaluation. In the presence of rhBMP-2, ectopic bone formation was found at 2 weeks and maturation of the newly formed bone around the composite at 8 weeks. Chitosan/HAp composite alone caused little inflammation, and new blood vessel growth and multinucleated giant cells were found around the composite, accompanied with roughening of the surface due to degradation at 2 weeks; however, neither cartilage nor bone formation was found around the composite. With rhBMP-2, the bioabsorption of the composite was accelerated as the rhBMP-2-induced bone matured. Histomorphometrical analysis showed that the mean value of the composite areas with rhBMP-2 was significantly smaller than that without rhBMT-2 at 2 and 8 weeks after the implantation. These results suggested that the novel chitosan/HAp composite was an effective bioabsorbable material as a carrier of rhBMP-2. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/44925 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 柏崎 晴彦
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