HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
Peer-reviewed Journal Articles, etc >

Monitoring intracellular degradation of exogenous DNA using diffusion properties

Files in This Item:
Sasaki main JCR_huscap.pdf623 kBPDFView/Open
Sasaki suppl JCR_huscap.pdf688.36 kBPDFView/Open
Please use this identifier to cite or link to this item:

Title: Monitoring intracellular degradation of exogenous DNA using diffusion properties
Authors: Sasaki, Akira Browse this author
Kinjo, Masataka Browse this author
Keywords: Fluorescence correlation spectroscopy
gene delivery
DNA stability
diffusion constant
Issue Date: Apr-2010
Publisher: Elsevier
Journal Title: Journal of Controlled Release
Volume: 143
Issue: 1
Start Page: 104
End Page: 111
Publisher DOI: 10.1016/j.jconrel.2009.12.013
PMID: 20035810
Abstract: Artificial nonviral gene vectors have the potential to improve the safety of gene therapy; however, such artificial vectors are less efficient for gene expression due to the existence of various barriers to delivery of exogenous DNAs to the nucleus in the living cell. Here we describe the degradation activities of cytoplasmic nucleases, which are involved as a barrier to efficient exogenous gene expression. The size and structure of degraded DNA were monitored by fluorescence correlation spectroscopy (FCS) and fluorescence cross-correlation spectroscopy (FCCS) in solution and in the cytoplasm of living cells. Differences in degradation by endo- and exonucleases were confirmed by differences in the size and structure of DNA. Moreover, we confirmed the influence of the exonuclease degradation in cytoplasm on the expression rate of DNA transfection with a cationic lipid. Based on a comparison of in vitro and cell measurements, we conclude that cytoplasmic degradation by exonucleases can be a considerable barrier against efficient gene delivery.
Type: article (author version)
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 佐々木 章

Export metadata:

OAI-PMH ( junii2 , jpcoar_1.0 )

MathJax is now OFF:


 - Hokkaido University