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Detection of all known filovirus species by reverse transcription-polymerase chain reaction using a primer set specific for the viral nucleoprotein gene

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Title: Detection of all known filovirus species by reverse transcription-polymerase chain reaction using a primer set specific for the viral nucleoprotein gene
Authors: Ogawa, Hirohito Browse this author
Miyamoto, Hiroko Browse this author
Ebihara, Hideki Browse this author
Ito, Kimihito Browse this author →KAKEN DB
Morikawa, Shigeru Browse this author →KAKEN DB
Feldmann, Heinz Browse this author
Takada, Ayato Browse this author →KAKEN DB
Keywords: Filovirus
Marburg virus
Ebola virus
Diagnosis
RT-PCR
Issue Date: Jan-2011
Publisher: Elsevier B.V.
Journal Title: Journal of Virological Methods
Volume: 171
Issue: 1
Start Page: 310
End Page: 313
Publisher DOI: 10.1016/j.jviromet.2010.11.010
Abstract: The filoviruses, Marburg virus (MARV) and Ebola virus (EBOV), are causative agents of severe hemorrhagic fever with high mortality rates in humans and non-human primates. Sporadic outbreaks of filovirus infection have occurred in Central Africa and parts of Asia. Identification of the natural reservoir animals that are unknown yet and epidemiological investigations are current challenges to forestall outbreaks of filovirus diseases. The filovirus species identified currently include one in the MARV group and five in the EBOV group, with large genetic variations found among the species. Therefore, it has been difficult to develop a single sensitive assay to detect all filovirus species, which would advance laboratory diagnosis greatly in endemic areas. In this study, a highly sensitive universal RT-PCR assay targeting the nucleoprotein (NP) gene of filoviruses was developed. The genomic RNAs of all known MARV and EBOV species were detected by using an NP-specific primer set. In addition, this RT-PCR procedure was verified further for its application to detect viral RNAs in tissue samples of animals infected experimentally and blood specimens of infected patients. This assay will be a useful method for diagnostics and epidemiological studies of filovirus infections.
Type: article (author version)
URI: http://hdl.handle.net/2115/44979
Appears in Collections:人獣共通感染症リサーチセンター (Research Center for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高田 礼人

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