|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Dental Medicine / Faculty of Dental Medicine >
Peer-reviewed Journal Articles, etc >
Regulation of CXCL12 expression by canonical Wnt signaling in bone marrow stromal cells
| Title: | Regulation of CXCL12 expression by canonical Wnt signaling in bone marrow stromal cells |
| Authors: | Tamura, Masato Browse this author | | Sato, Mari M. Browse this author | | Nashimoto, Masayuki Browse this author |
| Keywords: | CXCL12 | | Wnt | | Bone marrow stromal cells | | Osteoblast |
| Issue Date: | Mar-2011 |
| Publisher: | Elsevier |
| Journal Title: | The International Journal of Biochemistry & Cell Biology |
| Volume: | 43 |
| Issue: | 5 |
| Start Page: | 760 |
| End Page: | 767 |
| Publisher DOI: | 10.1016/j.biocel.2011.01.021 |
| PMID: | 21296678 |
| Abstract: | CXCL12 (stromal cell-derived factor-1, SDF-1), produced by stromal and endothelial cells including cells of the bone marrow, binds to its receptor CXCR4 and this axis regulates hematopoietic cell trafficking. Recently, osteoclast precursor cells were found to express CXCR4 and a potential role for the CXCL12–CXCR4 axis during osteoclast precursor cell recruitment/retention and development was proposed as a regulator of bone resorption. We examined the role of canonical Wnt signaling in regulating the expression of CXCL12 in bone marrow stromal cells. In mouse stromal ST2 cells, CXCL12 mRNA was expressed, while its expression was reduced in Wnt3a over-expressing ST2 (Wnt3a-ST2) cells or by treatment with lithium chloride (LiCl). Wnt3a decreased CXCL12 levels in culture supernatants from mouse bone marrow stromal cells. The culture supernatant from Wnt3a-ST2 cells also reduced migratory activity of bone marrow-derived cells in a Transwell migration assay. Silencing of glycogen synthase kinase-3β decreased CXCL12 expression, suggesting that the canonical Wnt signaling pathway regulates CXCL12 expression. In a transfection assay, LiCl down-regulated the activity of a reporter gene, a 1.8 kb fragment of the 5′-flanking region of the CXCL12 gene. These results show that canonical Wnt signaling regulates CXCL12 gene expression at the transcriptional level, and this is the first study linking chemokine expression to canonical Wnt signaling. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/45013 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 田村 正人
|
