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Deficiency of macrophage migration inhibitory factor gene delays healing of the medial collateral ligament : A biomechanical and biological study

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Title: Deficiency of macrophage migration inhibitory factor gene delays healing of the medial collateral ligament : A biomechanical and biological study
Authors: Kitayama, Soichiro Browse this author
Onodera, Shin Browse this author
Kondo, Eiji Browse this author
Kobayashi, Takumi Browse this author
Miyatake, Shin Browse this author
Kitamura, Nobuto Browse this author
Tohyama, Harukazu Browse this author
Yasuda, Kazunori Browse this author
Keywords: Medial collateral ligament
Macrophage migration inhibitory factor
Healing
Mouse
Matrix metalloproteinase
Issue Date: 3-Feb-2011
Publisher: Elsevier
Journal Title: Journal of Biomechanics
Volume: 44
Issue: 3
Start Page: 494
End Page: 500
Publisher DOI: 10.1016/j.jbiomech.2010.09.018
PMID: 20950810
Abstract: The role played by macrophage migration inhibitory factor (MIF) in the process of wound healing is controversial. Besides, there have been no reports that investigated the expression or the role of MIF in the repair process after ligament injury. In this study, we hypothesized that the deficiency in MIF gene might delay ligament healing in mice. The aim of this study was to clarify this hypothesis using MIF gene-deficient mice (MIFKO) and murine model of injury to the medial collateral ligament (MCL). Biomechanical testing showed that the levels of mechanical properties were significantly lower in MIFKO than in wild-type mice (WT) on day 28 after injury. Levels of matrix metalloproteinase (MMP)-2 and -13 mRNA in the healing tissue were significantly lower in MIFKO than in WT on day 28 and on day 7, respectively. Histologically, healing tissues in MIFKO exhibited prolonged hypertrophy, poor vascularity, and prolonged increase in cell number compared with those in WT. Taken together, it was suggested that MIFKO exhibited delayed healing of the MCL, which might be caused by lower mRNA expression of MMP-2 and -13.
Type: article (author version)
URI: http://hdl.handle.net/2115/45022
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小野寺 伸

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