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The Structure of Physarum polycephalum Hemagglutinin I Suggests a Minimal Carbohydrate Recognition Domain of Legume Lectin Fold

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Title: The Structure of Physarum polycephalum Hemagglutinin I Suggests a Minimal Carbohydrate Recognition Domain of Legume Lectin Fold
Authors: Kouno, Takahide Browse this author
Watanabe, Nobuhisa Browse this author
Sakai, Naoki Browse this author
Nakamura, Takashi Browse this author
Nabeshima, Yuko Browse this author
Morita, Masashi Browse this author
Mizuguchi, Mineyuki Browse this author
Aizawa, Tomoyasu Browse this author
Demura, Makoto Browse this author
Imanaka, Tsuneo Browse this author
Tanaka, Isao Browse this author
Kawano, Keiichi Browse this author
Keywords: carbohydrate recognition domain
jelly roll motif
lectin
β-sandwich fold
up-and-down β-sheet
Issue Date: 14-Jan-2011
Publisher: Elsevier
Journal Title: Journal of Molecular Biology
Volume: 405
Issue: 2
Start Page: 560
End Page: 569
Publisher DOI: 10.1016/j.jmb.2010.11.024
Abstract: Physarum polycephalum hemagglutinin I is a 104-residue protein that is secreted to extracellular space. The crystal structure of hemagglutinin I has a β-sandwich fold found among lectin structures, such as legume lectins and galectins. Interestingly, the β-sandwich of hemagglutinin I lacks a jelly roll motif and is essentially composed of two simple up-and-down β-sheets. This up-and-down β-sheet motif is well conserved in other lectins derived from animals, plants, bacteria, and viruses. It is more noteworthy that the up-and-down β-sheet motif includes many residues that make contact with the target carbohydrates. Our NMR data demonstrate that hemagglutinin I lacking a jelly roll motif also binds to its target glycopeptide. Taken together, the up-and-down β-sheet motif provides a fundamental scaffold for the binding of legume lectin-like proteins to the target carbohydrates, and the structure of hemagglutinin I suggests a minimal carbohydrate recognition domain.
Type: article (author version)
URI: http://hdl.handle.net/2115/45034
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 河野 敬一

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