Title: | The Structure of Physarum polycephalum Hemagglutinin I Suggests a Minimal Carbohydrate Recognition Domain of Legume Lectin Fold |
Authors: | Kouno, Takahide Browse this author |
Watanabe, Nobuhisa Browse this author |
Sakai, Naoki Browse this author |
Nakamura, Takashi Browse this author |
Nabeshima, Yuko Browse this author |
Morita, Masashi Browse this author |
Mizuguchi, Mineyuki Browse this author |
Aizawa, Tomoyasu Browse this author |
Demura, Makoto Browse this author |
Imanaka, Tsuneo Browse this author |
Tanaka, Isao Browse this author |
Kawano, Keiichi Browse this author |
Keywords: | carbohydrate recognition domain |
jelly roll motif |
lectin |
β-sandwich fold |
up-and-down β-sheet |
Issue Date: | 14-Jan-2011 |
Publisher: | Elsevier |
Journal Title: | Journal of Molecular Biology |
Volume: | 405 |
Issue: | 2 |
Start Page: | 560 |
End Page: | 569 |
Publisher DOI: | 10.1016/j.jmb.2010.11.024 |
Abstract: | Physarum polycephalum hemagglutinin I is a 104-residue protein that is secreted to extracellular space. The crystal structure of hemagglutinin I has a β-sandwich fold found among lectin structures, such as legume lectins and galectins. Interestingly, the β-sandwich of hemagglutinin I lacks a jelly roll motif and is essentially composed of two simple up-and-down β-sheets. This up-and-down β-sheet motif is well conserved in other lectins derived from animals, plants, bacteria, and viruses. It is more noteworthy that the up-and-down β-sheet motif includes many residues that make contact with the target carbohydrates. Our NMR data demonstrate that hemagglutinin I lacking a jelly roll motif also binds to its target glycopeptide. Taken together, the up-and-down β-sheet motif provides a fundamental scaffold for the binding of legume lectin-like proteins to the target carbohydrates, and the structure of hemagglutinin I suggests a minimal carbohydrate recognition domain. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/45034 |
Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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