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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
An HDAC inhibitor increases AcMNPV gene expression in mammalian cells
| Title: | An HDAC inhibitor increases AcMNPV gene expression in mammalian cells |
| Authors: | Fujita, Ryosuke Browse this author | | Ohtsuka, Daisuke Browse this author | | Sahara, Ken Browse this author | | Asano, Shinichiro Browse this author | | Bando, Hisanori Browse this author |
| Keywords: | Baculovirus | | AcMNPV | | Histone deacetylation | | Nucleosome | | Epigenetic | | Gene therapy |
| Issue Date: | Apr-2010 |
| Publisher: | Springer Wien |
| Journal Title: | Archives of Virology |
| Volume: | 155 |
| Issue: | 4 |
| Start Page: | 577 |
| End Page: | 581 |
| Publisher DOI: | 10.1007/s00705-010-0614-3 |
| PMID: | 20186445 |
| Abstract: | The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is used as a safer viral vector in mammalian cells with potential applications in gene therapy. However, the mechanism for the insusceptibility of mammalian cells to proliferative infection by entomopathogenic viruses is not well understood. Here, we studied the significance of epigenetic modifications such as histone acetylation, histone methylation and HP1 accumulation to AcMNPV gene expression in mammalian BHK cells. Real-time PCR and chromatin immunoprecipitation with sodium butyrate revealed an important relationship between viral gene expression and histone acetylation, having implications for a mechanism of suppression of AcMNPV gene expression in BHK cells. |
| Rights: | The original publication is available at www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/45094 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 伴戸 久徳
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