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Sexual dimorphism of gonadal structure and gene expression in germ cell-deficient loach, a teleost fish

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Title: Sexual dimorphism of gonadal structure and gene expression in germ cell-deficient loach, a teleost fish
Authors: Fujimoto, Takafumi Browse this author
Nishimura, Toshiya Browse this author
Goto-Kazeto, Rie Browse this author
Kawakami, Yutaka Browse this author
Yamaha, Etsuro Browse this author
Arai, Katsutoshi Browse this author
Keywords: dead end
gonadal development
sex differentiation
Issue Date: 5-Oct-2010
Publisher: National Academy of Sciences
Journal Title: Proceedings of the National Academy of Sciences of the United Stated of America
Volume: 107
Issue: 40
Start Page: 17211
End Page: 17216
Publisher DOI: 10.1073/pnas.1007032107
Abstract: Germ cell deficient fish usually develop as phenotypic males. Thus, the presence of germ cells is generally considered to be essential for female gonadal differentiation or the maintenance of ovarian structure. However, little is known of the role of germ cells in the determination of the sexual fate of gonadal somatic cells. We have established an inducible germ cell deficiency system in the loach (Misgurnus anguillicaudatus, Cypriniformes: Cobitidae), a small fresh-water fish, using knock-down of the dead end gene with a morpholino antisense oligonucleotide. Interestingly, loach lacking germ cells could develop as either phenotypic males or females, as characterized morphologically by the presence or absence of bony plates in the pectoral fins, respectively. The phenotypic males and females had testicular and ovarian structures, respectively, but lacked germ cells. Gene expression patterns in these male and female germ cell deficient gonads were essentially the same as those in gonads of normal fish. Our observations indicate that sexually dimorphic gonads can develop in germ cell deficient loach. In contrast to the situation in other model fish species, the gonadal somatic cells in phenotypic females autonomously differentiated into ovarian tissues and also played a role in the maintenance of gonadal structure. On the basis of our observations, we propose two possible models to explain the role of germ cells in sex determination in fish.
Type: article (author version)
URI: http://hdl.handle.net/2115/45100
Appears in Collections:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 荒井 克俊

 

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