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Sexual dimorphism of gonadal structure and gene expression in germ cell-deficient loach, a teleost fish
Title: | Sexual dimorphism of gonadal structure and gene expression in germ cell-deficient loach, a teleost fish |
Authors: | Fujimoto, Takafumi Browse this author | Nishimura, Toshiya Browse this author | Goto-Kazeto, Rie Browse this author | Kawakami, Yutaka Browse this author | Yamaha, Etsuro Browse this author →KAKEN DB | Arai, Katsutoshi Browse this author →KAKEN DB |
Keywords: | dead end | gonadal development | sex differentiation |
Issue Date: | 5-Oct-2010 |
Publisher: | National Academy of Sciences |
Journal Title: | Proceedings of the National Academy of Sciences of the United Stated of America |
Volume: | 107 |
Issue: | 40 |
Start Page: | 17211 |
End Page: | 17216 |
Publisher DOI: | 10.1073/pnas.1007032107 |
Abstract: | Germ cell deficient fish usually develop as phenotypic males. Thus, the presence of germ cells is generally considered to be essential for female gonadal differentiation or the maintenance of ovarian structure. However, little is known of the role of germ cells in the determination of the sexual fate of gonadal somatic cells. We have established an inducible germ cell deficiency system in the loach (Misgurnus anguillicaudatus, Cypriniformes: Cobitidae), a small fresh-water fish, using knock-down of the dead end gene with a morpholino antisense oligonucleotide. Interestingly, loach lacking germ cells could develop as either phenotypic males or females, as characterized morphologically by the presence or absence of bony plates in the pectoral fins, respectively. The phenotypic males and females had testicular and ovarian structures, respectively, but lacked germ cells. Gene expression patterns in these male and female germ cell deficient gonads were essentially the same as those in gonads of normal fish. Our observations indicate that sexually dimorphic gonads can develop in germ cell deficient loach. In contrast to the situation in other model fish species, the gonadal somatic cells in phenotypic females autonomously differentiated into ovarian tissues and also played a role in the maintenance of gonadal structure. On the basis of our observations, we propose two possible models to explain the role of germ cells in sex determination in fish. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/45100 |
Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 荒井 克俊
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