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The role of tumor necrosis factor-α for interleukin-10 production by murine dendritic cells
| Title: | The role of tumor necrosis factor-α for interleukin-10 production by murine dendritic cells |
| Authors: | Hirata, Noriyuki Browse this author | | Yanagawa, Yoshiki Browse this author | | Ogura, Hisako Browse this author | | Satoh, Masashi Browse this author | | Noguchi, Masayuki Browse this author | | Matsumoto, Machiko Browse this author | | Togashi, Hiroko Browse this author | | Onoé, Kazunori Browse this author | | Iwabuchi, Kazuya Browse this author |
| Keywords: | Dendritic cells | | Interleukin-10 | | Signal transduction | | Tumor necrosis factor-α | | Toll-like receptor |
| Issue Date: | 2011 |
| Publisher: | Elsevier |
| Journal Title: | Cellular Immunology |
| Volume: | 266 |
| Issue: | 2 |
| Start Page: | 165 |
| End Page: | 171 |
| Publisher DOI: | 10.1016/j.cellimm.2010.09.012 |
| PMID: | 20979991 |
| Abstract: | In the present study, we examined the role of tumor necrosis factor (TNF) in interleukin (IL)-10 production by dendritic cells (DCs) using bone-marrow derived DCs from wild type (WT) and TNF-α knockout (TNF-α^[-/-]) mice. Toll-like receptor (TLR) stimulation induced substantial level of IL-10 production by WT DCs, but significantly low level of IL-10 production by TNF-α^[-/-] DCs. In contrast, no significant difference was detected in IL-12 p40 production between WT and TNF-α^[-/-] DCs. Addition of TNF-α during TLR stimulation recovered the impaired ability of TNF-α^[-/-] DCs for IL-10 production. This recovery appeared to be associated with an activation of extracellular signal-regulated kinase, p38 mitogen-activated protein kinase, and phosphatidylinositol 3-kinase/Akt following the TNF-α addition. Blocking these kinases significantly inhibited IL-10 production by TNF-α^[-/-] DCs stimulated with TLR ligands plus TNF-α. Thus, TNF-α may be a key molecule to regulate the balance between anti-inflammatory versus inflammatory cytokine production in DCs. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/45137 |
| Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 平田 徳幸
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