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Failure of mycoplasma lipoprotein MALP-2 to induce NK cell activation through dendritic cell TLR2
Title: | Failure of mycoplasma lipoprotein MALP-2 to induce NK cell activation through dendritic cell TLR2 |
Authors: | Sawahata, Ryoko Browse this author | Shime, Hiroaki Browse this author | Yamazaki, Sayuri Browse this author | Inoue, Norimitsu Browse this author | Akazawa, Takashi Browse this author | Fujimoto, Yukari Browse this author | Fukase, Koichi Browse this author | Matsumoto, Misako Browse this author | Seya, Tsukasa Browse this author |
Keywords: | Toll-like receptor 2 | MyD88 | Macrophage-activating lipopeptide 2 | Dendritic cells | NK activation |
Issue Date: | Apr-2011 |
Publisher: | Elsevier Masson SAS |
Journal Title: | Microbes and Infection |
Volume: | 13 |
Issue: | 4 |
Start Page: | 350 |
End Page: | 358 |
Publisher DOI: | 10.1016/j.micinf.2010.12.003 |
PMID: | 21172450 |
Abstract: | Macrophage-activating lipopeptide 2 (MALP-2), a mycoplasmal diacylated lipopeptide with palmitic acid moiety (Pam2), activates Toll-like receptor (TLR) 2 to induce inflammatory cytokines. TLR2 is known to mature myeloid dendritic cells (mDC) to drive mDC contact-mediated natural killer (NK) cell activation. Here we tested if MALP-2 activates NK cells through stimulation of TLR2 on mDC. Although synthetic MALP-2 with 6 or 14 amino acids (a.a.) stretch (designated as s and f) matured mDC to induce IL-6, IL-12p40 and TNF-α to a similar extent, they far less activated NK cells than Pam2CSK4, a positive control of 6 a.a.-containing diacyl lipopeptide. MALP-2s and f were TLR2/6 agonists and activate the MyD88 pathway similar to Pam2CSK4, but MALP-2s having the CGNNDE sequence acted on mDC TLR2 to barely induce external NK activation. Even the s form, with slightly high induction of IL-6 compared to the f form, barely induced in vivo growth retardation of NK-sensitive implant tumor. Pam2CSK4 and MALP-2 have the common lipid moiety but different peptides, which are crucial for NK cell activation. The results infer that MALP-2 is applicable to a cytokine inducer but not to an adjuvant for antitumor NK immunotherapy. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/45255 |
Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 瀬谷 司
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