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Rapid discrimination and quantification of Theileria orientalis types using ribosomal DNA internal transcribed spacers

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タイトル: Rapid discrimination and quantification of Theileria orientalis types using ribosomal DNA internal transcribed spacers
著者: Kamau, Joseph 著作を一覧する
Salim, Bashir 著作を一覧する
Yokoyama, Naoki 著作を一覧する
Kinyanjui, Peter 著作を一覧する
Sugimoto, Chihiro 著作を一覧する
キーワード: Ribosomal RNA intergenic spacer
Theileria orientalis types
Genotyping
MPSP
発行日: 2011年 3月
出版者: Elsevier B.V.
誌名: Infection, Genetics and Evolution
巻: 11
号: 2
開始ページ: 407
終了ページ: 414
出版社 DOI: 10.1016/j.meegid.2010.11.016
抄録: We report the population structure analysis of Theileria orientalis types (Ikeda, Buffeli and Chitose), the causative agent of theileriosis in cattle and its cohorts, using ITS1 and ITS2 spacers by fragment genotyping. We utilized primers flanking the two ribosomal RNA internal transcribed spacers (ITS1 and ITS2). Due to varying degrees of sequence polymorphism in the ITS regions found within and between species, we exploited the insertions and or deletions in these regions which resulted in different fragment sizes. On the basis of fragment size polymorphism, we were able to discriminate the three commonly found types of Theileria orientalis. ITS1 was capable of discriminating all three types (Ikeda-251 bp, Chitose-274 bp and Buffeli-269 bp) in one single reaction by fragment genotyping. However, using ITS2, Ikeda (133-bp) a more pathogenic type was distinguishable from Buffeli/Chitose (139-bp). In addition, we quantified parasite load in experimental animals using ITS1. When compared with previous PCR detection method, ITS1 and ITS2 genotyping were found to be more sensitive methods with high specificity in population analysis and can be deployed in molecular epidemiology studies.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/45258
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 杉本 千尋

 

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