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Enzyme-Linked Immunosorbent Assay for Detection of Filovirus Species-Specific Antibodies
Title: | Enzyme-Linked Immunosorbent Assay for Detection of Filovirus Species-Specific Antibodies |
Authors: | Nakayama, Eri Browse this author | Yokoyama, Ayaka Browse this author | Miyamoto, Hiroko Browse this author | Igarashi, Manabu Browse this author →KAKEN DB | Kishida, Noriko Browse this author →KAKEN DB | Matsuno, Keita Browse this author | Marzi, Andrea Browse this author | Feldmann, Heinz Browse this author | Ito, Kimihito Browse this author →KAKEN DB | Saijo, Masayuki Browse this author →KAKEN DB | Takada, Ayato Browse this author →KAKEN DB |
Issue Date: | Nov-2010 |
Publisher: | American Society for Microbiology |
Journal Title: | Clinical and Vaccine Immunology |
Volume: | 17 |
Issue: | 11 |
Start Page: | 1723 |
End Page: | 1728 |
Publisher DOI: | 10.1128/CVI.00170-10 |
Abstract: | Several enzyme-linked immunosorbent assays (ELISA) for the detection of filovirus-specific antibodies have been developed. However, diagnostic methods to distinguish antibodies specific to the respective species of filoviruses, which provide the basis for serological classification, are not readily available. We established an ELISA using His-tagged secreted forms of the transmembrane glycoproteins (GPs) of five different Ebola virus (EBOV) species and one Marburg virus (MARV) strain as antigens for the detection of filovirus species-specific antibodies. The GP-based ELISA was evaluated by testing antisera collected from mice immunized with virus-like particles as well as from humans and nonhuman primates infected with EBOV or MARV. In our ELISA, little cross-reactivity of IgG antibodies was observed in most of the mouse antisera. Although sera and plasma from some patients and monkeys showed notable cross-reactivity with the GPs from multiple filovirus species, the highest reactions of IgG were uniformly detected against the GP antigen homologous to the virus species that infected individuals. We further confirmed that MARV-specific IgM antibodies were specifically detected in specimens collected from patients during the acute phase of infection. These results demonstrate the usefulness of our ELISA for diagnostics as well as ecological and serosurvey studies. |
Rights: | Copyright © 2010, American Society for Microbiology |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/45376 |
Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 高田 礼人
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