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Enzyme-Linked Immunosorbent Assay for Detection of Filovirus Species-Specific Antibodies

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Title: Enzyme-Linked Immunosorbent Assay for Detection of Filovirus Species-Specific Antibodies
Authors: Nakayama, Eri Browse this author
Yokoyama, Ayaka Browse this author
Miyamoto, Hiroko Browse this author
Igarashi, Manabu Browse this author →KAKEN DB
Kishida, Noriko Browse this author →KAKEN DB
Matsuno, Keita Browse this author
Marzi, Andrea Browse this author
Feldmann, Heinz Browse this author
Ito, Kimihito Browse this author →KAKEN DB
Saijo, Masayuki Browse this author →KAKEN DB
Takada, Ayato Browse this author →KAKEN DB
Issue Date: Nov-2010
Publisher: American Society for Microbiology
Journal Title: Clinical and Vaccine Immunology
Volume: 17
Issue: 11
Start Page: 1723
End Page: 1728
Publisher DOI: 10.1128/CVI.00170-10
Abstract: Several enzyme-linked immunosorbent assays (ELISA) for the detection of filovirus-specific antibodies have been developed. However, diagnostic methods to distinguish antibodies specific to the respective species of filoviruses, which provide the basis for serological classification, are not readily available. We established an ELISA using His-tagged secreted forms of the transmembrane glycoproteins (GPs) of five different Ebola virus (EBOV) species and one Marburg virus (MARV) strain as antigens for the detection of filovirus species-specific antibodies. The GP-based ELISA was evaluated by testing antisera collected from mice immunized with virus-like particles as well as from humans and nonhuman primates infected with EBOV or MARV. In our ELISA, little cross-reactivity of IgG antibodies was observed in most of the mouse antisera. Although sera and plasma from some patients and monkeys showed notable cross-reactivity with the GPs from multiple filovirus species, the highest reactions of IgG were uniformly detected against the GP antigen homologous to the virus species that infected individuals. We further confirmed that MARV-specific IgM antibodies were specifically detected in specimens collected from patients during the acute phase of infection. These results demonstrate the usefulness of our ELISA for diagnostics as well as ecological and serosurvey studies.
Rights: Copyright © 2010, American Society for Microbiology
Type: article (author version)
Appears in Collections:人獣共通感染症リサーチセンター (Research Center for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高田 礼人

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