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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Stabilizing effect of Ca2+ on myosin and myofibrils of squid mantle muscle as affected by heating conditions
| Title: | Stabilizing effect of Ca2+ on myosin and myofibrils of squid mantle muscle as affected by heating conditions |
| Authors: | Ohno, Takaaki Browse this author | | Kinoshita, Yasunori Browse this author | | Konno, Kunihiko Browse this author →KAKEN DB |
| Keywords: | Squid | | Myosin | | Denaturation | | Sorbitol | | Ca2+ |
| Issue Date: | May-2011 |
| Publisher: | Springer Japan |
| Journal Title: | Fisheries Science |
| Volume: | 77 |
| Issue: | 3 |
| Start Page: | 425 |
| End Page: | 430 |
| Publisher DOI: | 10.1007/s12562-011-0347-6 |
| Abstract: | Suppressive effect of Ca2+ on the thermal denaturation of myosin and on myofibrils of squid mantle muscle was compared. The stabilization effect on myosin was smaller than that on myofibrils, and was not affected by KCl concentration. The stabilizing effect of Ca2+ on myosin decreased with the lowering the temperature for heating showing no stabilization at 20℃, while the effect on myofibrils was evenly detected at all temperatures tested. Stabilizing effect of Ca2+ on myosin disappeared even at 30℃ in the presence of sorbitol showing a small inactivation rate, while the effect of Ca2+ was equally detected on myofibrils irrespective of the reduction in inactivation rate with sorbitol. Stabilization of myosin by Ca2+ again appeared even in the presence of sorbitol by raising the heating temperature to 38℃. It was suggested that Ca2+ confers stabilization on myosin only when myosin is in under unstable conditions. Stabilization effect of Ca2+ on myosin was enhanced upon F-actin binding, namely Ca2+ bound myosin was more significantly stabilized by F-actin binding, and the effect was no longer affected by the conditions for heating. |
| Rights: | © 2011 公益社団法人日本水産学会 | | The final publication is available at www.springerlink.com | | © 2011 The Japanese Society of Fisheries Science |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/45422 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 今野 久仁彦
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