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Stabilizing effect of Ca2+ on myosin and myofibrils of squid mantle muscle as affected by heating conditions

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Title: Stabilizing effect of Ca2+ on myosin and myofibrils of squid mantle muscle as affected by heating conditions
Authors: Ohno, Takaaki Browse this author
Kinoshita, Yasunori Browse this author
Konno, Kunihiko Browse this author →KAKEN DB
Keywords: Squid
Myosin
Denaturation
Sorbitol
Ca2+
Issue Date: May-2011
Publisher: Springer Japan
Journal Title: Fisheries Science
Volume: 77
Issue: 3
Start Page: 425
End Page: 430
Publisher DOI: 10.1007/s12562-011-0347-6
Abstract: Suppressive effect of Ca2+ on the thermal denaturation of myosin and on myofibrils of squid mantle muscle was compared. The stabilization effect on myosin was smaller than that on myofibrils, and was not affected by KCl concentration. The stabilizing effect of Ca2+ on myosin decreased with the lowering the temperature for heating showing no stabilization at 20℃, while the effect on myofibrils was evenly detected at all temperatures tested. Stabilizing effect of Ca2+ on myosin disappeared even at 30℃ in the presence of sorbitol showing a small inactivation rate, while the effect of Ca2+ was equally detected on myofibrils irrespective of the reduction in inactivation rate with sorbitol. Stabilization of myosin by Ca2+ again appeared even in the presence of sorbitol by raising the heating temperature to 38℃. It was suggested that Ca2+ confers stabilization on myosin only when myosin is in under unstable conditions. Stabilization effect of Ca2+ on myosin was enhanced upon F-actin binding, namely Ca2+ bound myosin was more significantly stabilized by F-actin binding, and the effect was no longer affected by the conditions for heating.
Rights: © 2011 公益社団法人日本水産学会
The final publication is available at www.springerlink.com
© 2011 The Japanese Society of Fisheries Science
Type: article (author version)
URI: http://hdl.handle.net/2115/45422
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 今野 久仁彦

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