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Membrane eicosapentaenoic acid is involved in the hydrophobicity of bacterial cells and affects the entry of hydrophilic and hydrophobic compounds

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Title: Membrane eicosapentaenoic acid is involved in the hydrophobicity of bacterial cells and affects the entry of hydrophilic and hydrophobic compounds
Authors: Nishida, Takanori Browse this author
Hori, Ryuji Browse this author
Morita, Naoki Browse this author
Okuyama, Hidetoshi Browse this author
Keywords: cell hydrophobicity
eicosapentaenoic acid
hydrogen peroxide
membrane-shielding effect
Shewanella
hydrophobic uncouplers
Issue Date: May-2010
Publisher: Blackwell Publishing
Journal Title: FEMS Microbiology Letters
Volume: 306
Issue: 2
Start Page: 91
End Page: 96
Publisher DOI: 10.1111/j.1574-6968.2010.01943.x
PMID: 20370838
Abstract: Eicosapentaenoic acid (EPA)-producing Shewanella marinintestina IK-1 (IK-1) and its EPA-deficient mutant IK-1Δ8 (IK-1Δ8) were grown on microtitre plates at 20 °C in a nutrient medium that contained various types of growth inhibitors. The minimal inhibitory concentrations of hydrogen peroxide and tert-butyl hydroxyl peroxide were 100 μM and 1 mM, respectively, for IK-1 and 10 and 100 μM, respectively, for IK-1Δ8. IK-1 was much more resistant than IK-1Δ8 to the four water-soluble antibiotics (ampicillin sodium, kanamycin sulphate, streptomycin sulphate, and tetracycline hydrochloride) tested. In contrast, IK-1 was less resistant than IK-1Δ8 to two hydrophobic uncouplers: carbonyl cyanide m-chloro phenylhydrazone (CCCP) and N,N′-dicyclohexylcarbodiimide (DCCD). The hydrophobicity of the IK-1 and IK-1Δ8 cells grown at 20 °C was determined using the bacterial adhesion to hydrocarbon method. EPA-containing (∼10% of total fatty acids) IK-1 cells were more hydrophobic than their counterparts with no EPA. These results suggest that the high hydrophobicity of IK-1 cells can be attributed to the presence of membrane EPA, which shields the entry of hydrophilic membrane-diffusible compounds, and that hydrophobic compounds such as CCCP and DCCD diffuse more effectively in the membranes of IK-1, where they can fulfil their inhibitory activities, than in the membranes of IK-1Δ8.
Rights: The definitive version is available at www.blackwell-synergy.com
Type: article (author version)
URI: http://hdl.handle.net/2115/45472
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 奥山 英登志

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