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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Design of Sphingomonad-Detecting Probes for a DNA Array, and Its Application to Investigate the Behavior, Distribution, and Source of Rhizospherous Sphingomonas and Other Sphingomonads Inhabiting an Acid Sulfate Soil Paddock in Kalimantan, Indonesia
| Title: | Design of Sphingomonad-Detecting Probes for a DNA Array, and Its Application to Investigate the Behavior, Distribution, and Source of Rhizospherous Sphingomonas and Other Sphingomonads Inhabiting an Acid Sulfate Soil Paddock in Kalimantan, Indonesia |
| Authors: | HASHIDOKO, Yasuyuki Browse this author | | KITAGAWA, Emiko Browse this author | | IWAHASHI, Hitoshi Browse this author | | PURNOMO, Erry Browse this author | | HASEGAWA, Toshihiro Browse this author | | TAHARA, Satoshi Browse this author |
| Keywords: | acid sulfate soil paddock | | Juncus sp | | source of rhizospherous sphingomonads | | Sphingomonas-detecting DNA array probes |
| Issue Date: | 23-Feb-2007 |
| Publisher: | 日本農芸化学会 |
| Journal Title: | Bioscience, Biotechnology, and Biochemistry |
| Volume: | 71 |
| Issue: | 2 |
| Start Page: | 343 |
| End Page: | 351 |
| Publisher DOI: | 10.1271/bbb.60262 |
| PMID: | 17284854 |
| Abstract: | Throughout Central and South Kalimantan, Indonesia, strongly acidic soil (pH 2.1–3.7) is widely distributed, and the local acidic soil-tolerant plants, including local rice varieties, often possess sphingomonads in their rhizosphere and rhizoplane. To investigate the behavior of sphingomonads inhabiting the rhizosphere of such acid-tolerant plants, we designed 13 different DNA array probes (each of 72 mer) specific to a group of sphingomonads, using a hypervariable V6 region of the 16S rRNA gene. This DNA array system was used preliminarily for an analysis of microfloral dynamisms, particularly of sphingomonads, in acidic paddock ecosystems, and the results suggest that the acid-tolerant local rice shares rhizospherous sphingomonads with wild Juncus sp., a predominant weed that thrives in acidic paddocks during the off-season for rice farming. This tentative conclusion supports the bio-rationality of the traditional rice farming system with respect to functional rhizobacteria. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/45491 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 橋床 泰之
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