HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >

Identification of the 4-Hydroxycinnamate Decarboxylase (PAD) Gene of Klebsiella oxytoca

Files in This Item:
hashidoko4.pdf376.76 kBPDFView/Open
Please use this identifier to cite or link to this item:

Title: Identification of the 4-Hydroxycinnamate Decarboxylase (PAD) Gene of Klebsiella oxytoca
Authors: UCHIYAMA, Hirofumi Browse this author
HASHIDOKO, Yasuyuki Browse this author
KURIYAMA, Yuhki Browse this author
TAHARA, Satoshi Browse this author
Keywords: Klebsiella oxytoca
bacterial Pad gene
PCR detection of Pad
Gram-negative bacteria
4-hydroxycinnamate decarboxylase gene
Issue Date: 23-Jan-2008
Publisher: 日本農芸化学会
Journal Title: Bioscience, Biotechnology, and Biochemistry
Volume: 72
Issue: 1
Start Page: 116
End Page: 123
Publisher DOI: 10.1271/bbb.70496
PMID: 18175898
Abstract: A 7.1-kbp DNA fragment isolated from a wild strain of Klebsiella oxytoca was sequenced, leading to the identification of 10 open-reading frames (ORFs), including a 504-bp Pad gene. The Pad gene of the Gram-negative bacterium was subsequently expressed in Escherichia coli as a chimeric Pad. The deduced amino acid (AA) sequence of the Pad gene from wild-type K. oxytoca showed approximately 50% homology to those of other bacterial PADs from Gram-positive bacilli plus a coccus. These data and a genomic library search of some γ-proteobacteria, including E. coli and Vibrio sp., indicated that PAD of K. oxytoca is a member of the bacterial PAD family characteristic of Gram-negative bacteria. Using Pad-specific PCR primers designed from the Gram-negative bacterial Pad of K. oxytoca, Pad genes of two further strains of K. oxytoca, another wild isolate and JCM 1665 and two PAD-positive Enterobacter spp. were successfully amplified for specific Pad detection.
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 橋床 泰之

Export metadata:

OAI-PMH ( junii2 , jpcoar_1.0 )

MathJax is now OFF:


 - Hokkaido University