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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Identification of the 4-Hydroxycinnamate Decarboxylase (PAD) Gene of Klebsiella oxytoca
| Title: | Identification of the 4-Hydroxycinnamate Decarboxylase (PAD) Gene of Klebsiella oxytoca |
| Authors: | UCHIYAMA, Hirofumi Browse this author | | HASHIDOKO, Yasuyuki Browse this author | | KURIYAMA, Yuhki Browse this author | | TAHARA, Satoshi Browse this author |
| Keywords: | Klebsiella oxytoca | | bacterial Pad gene | | PCR detection of Pad | | Gram-negative bacteria | | 4-hydroxycinnamate decarboxylase gene |
| Issue Date: | 23-Jan-2008 |
| Publisher: | 日本農芸化学会 |
| Journal Title: | Bioscience, Biotechnology, and Biochemistry |
| Volume: | 72 |
| Issue: | 1 |
| Start Page: | 116 |
| End Page: | 123 |
| Publisher DOI: | 10.1271/bbb.70496 |
| PMID: | 18175898 |
| Abstract: | A 7.1-kbp DNA fragment isolated from a wild strain of Klebsiella oxytoca was sequenced, leading to the identification of 10 open-reading frames (ORFs), including a 504-bp Pad gene. The Pad gene of the Gram-negative bacterium was subsequently expressed in Escherichia coli as a chimeric Pad. The deduced amino acid (AA) sequence of the Pad gene from wild-type K. oxytoca showed approximately 50% homology to those of other bacterial PADs from Gram-positive bacilli plus a coccus. These data and a genomic library search of some γ-proteobacteria, including E. coli and Vibrio sp., indicated that PAD of K. oxytoca is a member of the bacterial PAD family characteristic of Gram-negative bacteria. Using Pad-specific PCR primers designed from the Gram-negative bacterial Pad of K. oxytoca, Pad genes of two further strains of K. oxytoca, another wild isolate and JCM 1665 and two PAD-positive Enterobacter spp. were successfully amplified for specific Pad detection. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/45492 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 橋床 泰之
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