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Isolation of BAC Clones Containing Conserved Genes from Libraries of Three Distantly Related Moths : A Useful Resource for Comparative Genomics of Lepidoptera

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Title: Isolation of BAC Clones Containing Conserved Genes from Libraries of Three Distantly Related Moths : A Useful Resource for Comparative Genomics of Lepidoptera
Authors: Yasukochi, Yuji Browse this author
Tanaka-Okuyama, Makiko Browse this author
Kamimura, Manabu Browse this author
Nakano, Ryo Browse this author
Naito, Yota Browse this author
Ishikawa, Yukio Browse this author
Sahara, Ken Browse this author
Issue Date: 2011
Publisher: Hindawi Publishing Corporation
Journal Title: Journal of Biomedicine and Biotechnology
Volume: 2011
Start Page: 165894
Publisher DOI: 10.1155/2011/165894
Abstract: Lepidoptera, butterflies and moths, is the second largest animal order and includes numerous agricultural pests. To facilitate comparative genomics in Lepidoptera, we isolated BAC clones containing conserved and putative single-copy genes from libraries of three pests, Heliothis virescens, Ostrinia nubilalis, and Plutella xylostella, harboring the haploid chromosome number, n = 31, which are not closely related with each other or with the silkworm, Bombyx mori, (n = 28), the sequenced model lepidopteran. A total of 108-184 clones representing 101-182 conserved genes were isolated for each species. For 79 genes, clones were isolated from more than two species, which will be useful as common markers for analysis using fluorescence in situ hybridization (FISH), as well as for comparison of genome sequence among multiple species. The PCR-based clone isolation method presented here is applicable to species which lack a sequenced genome but have a significant collection of cDNA or EST sequences.
Rights: http://creativecommons.org/licenses/by/3.0/
Type: article
URI: http://hdl.handle.net/2115/45606
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 佐原 健

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