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Gene and protein analysis of brain derived neurotrophic factor expression in relation to neurological recovery induced by an enriched environment in a rat stroke model
| Title: | Gene and protein analysis of brain derived neurotrophic factor expression in relation to neurological recovery induced by an enriched environment in a rat stroke model |
| Authors: | Hirata, Kenji Browse this author | | Kuge, Yuji Browse this author | | Yokota, Chiaki Browse this author | | Harada, Akina Browse this author | | Kokame, Koichi Browse this author | | Inoue, Hiroyasu Browse this author | | Kawashima, Hidekazu Browse this author | | Hanzawa, Hiroko Browse this author | | Shono, Yuji Browse this author | | Saji, Hideo Browse this author | | Minematsu, Kazuo Browse this author | | Tamaki, Nagara Browse this author |
| Keywords: | Focal ischemia | | Enriched environment | | Brain derived neurotrophic factor |
| Issue Date: | 20-May-2011 |
| Publisher: | Elsevier Ireland |
| Journal Title: | Neuroscience Letters |
| Volume: | 495 |
| Issue: | 3 |
| Start Page: | 210 |
| End Page: | 215 |
| Publisher DOI: | 10.1016/j.neulet.2011.03.068 |
| PMID: | 21457756 |
| Abstract: | Although an enriched environment enhances functional recovery after ischemic stroke, the mechanism underlying this effect remains unclear. We previously reported that brain derived neurotrophic factor (BDNF) gene expression decreased in rats housed in an enriched environment for 4 weeks compared to those housed in a standard cage for the same period. To further clarify the relationship between the decrease in BDNF and functional recovery, we investigated the effects of differential 2-week housing conditions on the mRNA of BDNF and protein levels of proBDNF and mature BDNF (matBDNF). After transient occlusion of the right middle cerebral artery of male Sprague-Dawley rats, we divided the rats into two groups: (1) an enriched group housed multiply in large cages equipped with toys, and (2) a standard group housed alone in small cages without toys. Behavioral tests before and after 2-week differential housing showed better neurological recovery in the enriched group than in the standard group. Synaptophysin immunostaining demonstrated that the density of synapses in the peri-infarct area was increased in the enriched group compared to the standard group, while infarct volumes were not significantly different. Real-time reverse transcription polymerase chain reaction, Western blotting and immunostaining all revealed no significant difference between the groups. The present results suggest that functional recovery cannot be ascribed to an increase in matBDNF or a decrease in proBDNF but rather to other underlying mechanisms. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/45814 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 平田 健司
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