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Gene and protein analysis of brain derived neurotrophic factor expression in relation to neurological recovery induced by an enriched environment in a rat stroke model

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Title: Gene and protein analysis of brain derived neurotrophic factor expression in relation to neurological recovery induced by an enriched environment in a rat stroke model
Authors: Hirata, Kenji Browse this author
Kuge, Yuji Browse this author
Yokota, Chiaki Browse this author
Harada, Akina Browse this author
Kokame, Koichi Browse this author
Inoue, Hiroyasu Browse this author
Kawashima, Hidekazu Browse this author
Hanzawa, Hiroko Browse this author
Shono, Yuji Browse this author
Saji, Hideo Browse this author
Minematsu, Kazuo Browse this author
Tamaki, Nagara Browse this author
Keywords: Focal ischemia
Enriched environment
Brain derived neurotrophic factor
Issue Date: 20-May-2011
Publisher: Elsevier Ireland
Journal Title: Neuroscience Letters
Volume: 495
Issue: 3
Start Page: 210
End Page: 215
Publisher DOI: 10.1016/j.neulet.2011.03.068
PMID: 21457756
Abstract: Although an enriched environment enhances functional recovery after ischemic stroke, the mechanism underlying this effect remains unclear. We previously reported that brain derived neurotrophic factor (BDNF) gene expression decreased in rats housed in an enriched environment for 4 weeks compared to those housed in a standard cage for the same period. To further clarify the relationship between the decrease in BDNF and functional recovery, we investigated the effects of differential 2-week housing conditions on the mRNA of BDNF and protein levels of proBDNF and mature BDNF (matBDNF). After transient occlusion of the right middle cerebral artery of male Sprague-Dawley rats, we divided the rats into two groups: (1) an enriched group housed multiply in large cages equipped with toys, and (2) a standard group housed alone in small cages without toys. Behavioral tests before and after 2-week differential housing showed better neurological recovery in the enriched group than in the standard group. Synaptophysin immunostaining demonstrated that the density of synapses in the peri-infarct area was increased in the enriched group compared to the standard group, while infarct volumes were not significantly different. Real-time reverse transcription polymerase chain reaction, Western blotting and immunostaining all revealed no significant difference between the groups. The present results suggest that functional recovery cannot be ascribed to an increase in matBDNF or a decrease in proBDNF but rather to other underlying mechanisms.
Type: article (author version)
URI: http://hdl.handle.net/2115/45814
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 平田 健司

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