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Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51
| Title: | Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51 |
| Authors: | Ochiai, Kazuhiko Browse this author | | Yoshikawa, Yasunaga Browse this author | | Yoshimatsu, Kumiko Browse this author | | Oonuma, Toshina Browse this author | | Tomioka, Yukiko Browse this author | | Takeda, Eichi Browse this author | | Arikawa, Jiro Browse this author | | Mominoki, Katsumi Browse this author | | Omi, Toshinori Browse this author | | Hashizume, Kazuyoshi Browse this author | | Morimatsu, Masami Browse this author |
| Keywords: | BRCA2 | | RAD51 | | BRC repeat | | Homologous recombination | | Canine |
| Issue Date: | 23-Jun-2011 |
| Publisher: | Elsevier B.V. |
| Journal Title: | FEBS Letters |
| Volume: | 585 |
| Issue: | 12 |
| Start Page: | 1771 |
| End Page: | 1777 |
| Publisher DOI: | 10.1016/j.febslet.2011.05.027 |
| Abstract: | The breast cancer susceptibility protein BRCA2 is essential for recombinational DNA repair. BRCA2 specifically binds to RAD51 via 8 BRC repeat motifs and delivers RAD51 to double-stranded DNA breaks. In this study, a mammalian two-hybrid assay and competitive ELISA showed that the interaction between BRC repeat 4 (BRC4) and RAD51 was strengthened by the substitution of a single BRC4 amino acid from valine to isoleucine (V1532I). However, the cancer-associated V1532F mutant exhibited very weak interaction with RAD51. This study used a comparative analysis of BRC4 between animal species to identify V1532 as an important residue that interacts with RAD51. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/45957 |
| Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 森松 正美
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