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Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51

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Title: Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51
Authors: Ochiai, Kazuhiko Browse this author
Yoshikawa, Yasunaga Browse this author
Yoshimatsu, Kumiko Browse this author
Oonuma, Toshina Browse this author
Tomioka, Yukiko Browse this author
Takeda, Eichi Browse this author
Arikawa, Jiro Browse this author
Mominoki, Katsumi Browse this author
Omi, Toshinori Browse this author
Hashizume, Kazuyoshi Browse this author
Morimatsu, Masami Browse this author
Keywords: BRCA2
BRC repeat
Homologous recombination
Issue Date: 23-Jun-2011
Publisher: Elsevier B.V.
Journal Title: FEBS Letters
Volume: 585
Issue: 12
Start Page: 1771
End Page: 1777
Publisher DOI: 10.1016/j.febslet.2011.05.027
Abstract: The breast cancer susceptibility protein BRCA2 is essential for recombinational DNA repair. BRCA2 specifically binds to RAD51 via 8 BRC repeat motifs and delivers RAD51 to double-stranded DNA breaks. In this study, a mammalian two-hybrid assay and competitive ELISA showed that the interaction between BRC repeat 4 (BRC4) and RAD51 was strengthened by the substitution of a single BRC4 amino acid from valine to isoleucine (V1532I). However, the cancer-associated V1532F mutant exhibited very weak interaction with RAD51. This study used a comparative analysis of BRC4 between animal species to identify V1532 as an important residue that interacts with RAD51.
Type: article (author version)
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 森松 正美

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