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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Medicine / Faculty of Medicine >
Peer-reviewed Journal Articles, etc >
Development of Mouse Hepatocyte Lines Permissive for Hepatitis C Virus (HCV)
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| Title: | Development of Mouse Hepatocyte Lines Permissive for Hepatitis C Virus (HCV) |
| Authors: | Aly, Hussein Hassan Browse this author | | Oshiumi, Hiroyuki Browse this author | | Shime, Hiroaki Browse this author | | Matsumoto, Misako Browse this author | | Wakita, Taka Browse this author | | Shimotohno, Kunitada Browse this author | | Seya, Tsukasa Browse this author |
| Issue Date: | 22-Jun-2011 |
| Publisher: | Public Library of Science |
| Journal Title: | PLoS One |
| Volume: | 6 |
| Issue: | 6 |
| Start Page: | e21284 |
| Publisher DOI: | 10.1371/journal.pone.0021284 |
| Abstract: | The lack of a suitable small animal model for the analysis of hepatitis C virus (HCV) infection has hampered elucidation of the HCV life cycle and the development of both protective and therapeutic strategies against HCV infection. Human and mouse harbor a comparable system for antiviral type I interferon (IFN) induction and amplification, which regulates viral infection and replication. Using hepatocytes from knockout (ko) mice, we determined the critical step of the IFN-inducing/ amplification pathways regulating HCV replication in mouse. The results infer that interferon-beta promoter stimulator (IPS1) or interferon A receptor (IFNAR) were a crucial barrier to HCV replication in mouse hepatocytes. Although both IFNARko and IPS-1ko hepatocytes showed a reduced induction of type I interferons in response to viral infection, only IPS-1-/- cells circumvented cell death from HCV cytopathic effect and significantly improved J6JFH1 replication, suggesting IPS-1 to be a key player regulating HCV replication in mouse hepatocytes. We then established mouse hepatocyte lines lacking IPS-1 or IFNAR through immortalization with SV40T antigen. Expression of human (h)CD81 on these hepatocyte lines rendered both lines HCVcc-permissive. We also found that the chimeric J6JFH1 construct, having the structure region from J6 isolate enhanced HCV replication in mouse hepatocytes rather than the full length original JFH1 construct, a new finding that suggests the possible role of the HCV structural region in HCV replication. This is the first report on the entry and replication of HCV infectious particles in mouse hepatocytes. These mouse hepatocyte lines will facilitate establishing a mouse HCV infection model with multifarious applications. |
| Rights: | http://creativecommons.org/licenses/by/2.5/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/46796 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 瀬谷 司
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