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Volume 8, Number 1-4  >

PURIFICATION STUDIES ON THE INFECTIOUS CANINE HEPATITIS VIRUS : I. VIRAL PARTICLES IN TISSUE CULTURE FLUID REVEALED BY ONE PURIFICATION PROCEDURE

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.8.1-4.208

Title: PURIFICATION STUDIES ON THE INFECTIOUS CANINE HEPATITIS VIRUS : I. VIRAL PARTICLES IN TISSUE CULTURE FLUID REVEALED BY ONE PURIFICATION PROCEDURE
Authors: KUNISHIGE, Teruo Browse this author
SHIMIZU, Yukio Browse this author
HIRATO, Katsushichi Browse this author
Issue Date: 1960
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 8
Issue: 1-4
Start Page: 208
End Page: 220
Abstract: Infectious canine hepatitis virus, strain "MATSUDA", from dog kidney tissue culture has been purified by methanol precipitation, extraction with organic solvents, and differential centrifugation. The ICH virus particle in the resulting purified virus concentrates has been identified by electron microscopy in agar pseudo-replica specimens as a uniform-sized particle 85mμ (unshadow), 96mμ (Pd-shadow) in diameter. These particles were found in parallel with viral infectivity. Some of the particles contain a central dense core surrounded by less dense material enclosed in a membrane when unshadowed and this central core when shadowed appears to be protuberant.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/4680
Appears in Collections:Japanese Journal of Veterinary Research > Volume 8, Number 1-4

Submitter: 獣医学部図書室

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