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Paradoxical effects of chondroitin sulfate-E on Japanese encephalitis viral infection
| Title: | Paradoxical effects of chondroitin sulfate-E on Japanese encephalitis viral infection |
| Authors: | Kim, Eunmi Browse this author | | Okumura, Megumi Browse this author | | Sawa, Hirofumi Browse this author | | Miyazaki, Tadaaki Browse this author →KAKEN DB | | Fujikura, Daisuke Browse this author | | Yamada, Shuhei Browse this author | | Sugahara, Kazuyuki Browse this author | | Sasaki, Michihito Browse this author | | Kimura, Takashi Browse this author |
| Keywords: | Chondroitin sulfate E | | Japanese encephalitis virus | | Neural cell |
| Issue Date: | 17-Jun-2011 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 409 |
| Issue: | 4 |
| Start Page: | 717 |
| End Page: | 722 |
| Publisher DOI: | 10.1016/j.bbrc.2011.05.072 |
| PMID: | 21621516 |
| Abstract: | Glycosaminoglycans (GAGs) have diverse functions in the body and are involved in viral infection. The purpose of this study was to evaluate the possible roles of the E-disaccharide units GlcAβ1-3GalNAc(4,6-O-disulfate) of chondroitin sulfate (CS), a GAG involved in neuritogenesis and neuronal migration, in Japanese encephalitis virus (JEV) infection. Soluble CS-E (sCS-E) derived from squid cartilage inhibited JEV infection in African green monkey kidney-derived Vero cells and baby hamster kidney-derived BHK cells by interfering with viral attachment. In contrast, sCS-E enhanced viral infection in the mouse neuroblastoma cell line Neuro-2a, despite the fact that viral attachment to Neuro-2a cells was inhibited by sCS-E. This enhancement effect in Neuro-2a cells seemed to be related to increased viral RNA replication and was also observed in a rat infection model in which intracerebral coadministration of sCS-E with JEV in 17-day-old rats resulted in higher brain viral loads than in rats infected without sCS-E administration. These results show the paradoxical effects of sCS-E on JEV infection in different cell types and indicate that potential use of sCS-E as an antiviral agent against JEV infection should be approached with caution considering its effects in the neuron, the major target of JEV. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/46843 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 木村 享史
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