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Paradoxical effects of chondroitin sulfate-E on Japanese encephalitis viral infection

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Title: Paradoxical effects of chondroitin sulfate-E on Japanese encephalitis viral infection
Authors: Kim, Eunmi Browse this author
Okumura, Megumi Browse this author
Sawa, Hirofumi Browse this author
Miyazaki, Tadaaki Browse this author →KAKEN DB
Fujikura, Daisuke Browse this author
Yamada, Shuhei Browse this author
Sugahara, Kazuyuki Browse this author
Sasaki, Michihito Browse this author
Kimura, Takashi Browse this author
Keywords: Chondroitin sulfate E
Japanese encephalitis virus
Neural cell
Issue Date: 17-Jun-2011
Publisher: Elsevier
Journal Title: Biochemical and Biophysical Research Communications
Volume: 409
Issue: 4
Start Page: 717
End Page: 722
Publisher DOI: 10.1016/j.bbrc.2011.05.072
PMID: 21621516
Abstract: Glycosaminoglycans (GAGs) have diverse functions in the body and are involved in viral infection. The purpose of this study was to evaluate the possible roles of the E-disaccharide units GlcAβ1-3GalNAc(4,6-O-disulfate) of chondroitin sulfate (CS), a GAG involved in neuritogenesis and neuronal migration, in Japanese encephalitis virus (JEV) infection. Soluble CS-E (sCS-E) derived from squid cartilage inhibited JEV infection in African green monkey kidney-derived Vero cells and baby hamster kidney-derived BHK cells by interfering with viral attachment. In contrast, sCS-E enhanced viral infection in the mouse neuroblastoma cell line Neuro-2a, despite the fact that viral attachment to Neuro-2a cells was inhibited by sCS-E. This enhancement effect in Neuro-2a cells seemed to be related to increased viral RNA replication and was also observed in a rat infection model in which intracerebral coadministration of sCS-E with JEV in 17-day-old rats resulted in higher brain viral loads than in rats infected without sCS-E administration. These results show the paradoxical effects of sCS-E on JEV infection in different cell types and indicate that potential use of sCS-E as an antiviral agent against JEV infection should be approached with caution considering its effects in the neuron, the major target of JEV.
Type: article (author version)
Appears in Collections:人獣共通感染症リサーチセンター (Research Center for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 木村 享史

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