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Paradoxical effects of chondroitin sulfate-E on Japanese encephalitis viral infection

BBC409-4_717-722.pdf662.03 kBPDF見る/開く

タイトル: Paradoxical effects of chondroitin sulfate-E on Japanese encephalitis viral infection
著者: Kim, Eunmi 著作を一覧する
Okumura, Megumi 著作を一覧する
Sawa, Hirofumi 著作を一覧する
Miyazaki, Tadaaki 著作を一覧する
Fujikura, Daisuke 著作を一覧する
Yamada, Shuhei 著作を一覧する
Sugahara, Kazuyuki 著作を一覧する
Sasaki, Michihito 著作を一覧する
Kimura, Takashi 著作を一覧する
キーワード: Chondroitin sulfate E
Japanese encephalitis virus
Neural cell
発行日: 2011年 6月17日
出版者: Elsevier
誌名: Biochemical and Biophysical Research Communications
巻: 409
号: 4
開始ページ: 717
終了ページ: 722
出版社 DOI: 10.1016/j.bbrc.2011.05.072
抄録: Glycosaminoglycans (GAGs) have diverse functions in the body and are involved in viral infection. The purpose of this study was to evaluate the possible roles of the E-disaccharide units GlcAβ1-3GalNAc(4,6-O-disulfate) of chondroitin sulfate (CS), a GAG involved in neuritogenesis and neuronal migration, in Japanese encephalitis virus (JEV) infection. Soluble CS-E (sCS-E) derived from squid cartilage inhibited JEV infection in African green monkey kidney-derived Vero cells and baby hamster kidney-derived BHK cells by interfering with viral attachment. In contrast, sCS-E enhanced viral infection in the mouse neuroblastoma cell line Neuro-2a, despite the fact that viral attachment to Neuro-2a cells was inhibited by sCS-E. This enhancement effect in Neuro-2a cells seemed to be related to increased viral RNA replication and was also observed in a rat infection model in which intracerebral coadministration of sCS-E with JEV in 17-day-old rats resulted in higher brain viral loads than in rats infected without sCS-E administration. These results show the paradoxical effects of sCS-E on JEV infection in different cell types and indicate that potential use of sCS-E as an antiviral agent against JEV infection should be approached with caution considering its effects in the neuron, the major target of JEV.
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 木村 享史


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