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Volume 32, Number 2 >

LABELED AVIDIN-BIOTIN ENZYME-LINKED IMMUNOSORBENT ASSAY (LAB-ELISA) FOR DETECTION OF JAPANESE ENCEPHALITIS ANTIBODY IN SWINE SERA

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.32.2.59

Title: LABELED AVIDIN-BIOTIN ENZYME-LINKED IMMUNOSORBENT ASSAY (LAB-ELISA) FOR DETECTION OF JAPANESE ENCEPHALITIS ANTIBODY IN SWINE SERA
Authors: CHANG, Hsi-Chi Browse this author
OHKUBO, Yukihiro Browse this author
TAKASHIMA, Ikuo Browse this author
ARIKAWA, Jiro Browse this author
HASHIMOTO, Nobuo Browse this author
Keywords: LAB-ELISA
avidin-biotin
Japanese encephalitis
swine
Issue Date: 28-Apr-1984
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 32
Issue: 2
Start Page: 59
End Page: 71
Abstract: Labeled avidin-biotin (LAB) enzyme-linked immunosorbent assay (ELISA) was applied to detect Japanese encephalitis (JE) antibody in swine sera. Purified JaGAr-01 vaccine of JE, biotin labeled anti-swine Ig or IgM rabbit IgG conjugate and horseradish peroxidase (HRP) were employed in the assay. The μ chain specific anti-IgM conjugate could detect only IgM antibody, but the anti-Ig conjugate could detect both IgM and IgG antibodibes to JE. When 2-2'-azino di-[3-ethylbenzthiazoline sulphonic acid](ABTS) was used as a substrate to HRP, sensitivity of LAB-ELISA was essentially the same as that of ordinary ELISA, but approximately 20 times higher than that of hemagglutination inhibition (HI) test. LAB-ELISA titers with ABTS were approximately 5 times higher than those with 5-amino salcylic acid (5-AS). However, non-specific reactions of sera were about 4 times lower in LAB-ELISA than in ordinary ELISA.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/4693
Appears in Collections:Japanese Journal of Veterinary Research > Volume 32, Number 2

Submitter: 獣医学部図書室

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