HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Medicine / Faculty of Medicine >
Peer-reviewed Journal Articles, etc >

A population of BJ fibroblasts escaped from Ras-induced senescence susceptible to transformation

Files in This Item:
BBRC410-4_878-884.pdf672.01 kBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/46956

Title: A population of BJ fibroblasts escaped from Ras-induced senescence susceptible to transformation
Authors: Kohsaka, Shinji Browse this author
Sasai, Ken Browse this author
Takahashi, Kenta Browse this author
Akagi, Tsuyoshi Browse this author
Tanino, Mishie Browse this author
Kimura, Taichi Browse this author
Nishihara, Hiroshi Browse this author
Tanaka, Shinya Browse this author
Keywords: Oncogene-induced senescence
Ras
p16
p53
Stem cell
Issue Date: 15-Jul-2011
Publisher: Elsevier
Journal Title: Biochemical and Biophysical Research Communications
Volume: 410
Issue: 4
Start Page: 878
End Page: 884
Publisher DOI: 10.1016/j.bbrc.2011.06.082
PMID: 21703241
Abstract: Oncogenic stimuli such as H-Ras induce oncogene-induced senescence (OIS) in fibroblasts to protect against transformation. Here we found that a population of the human diploid fibroblasts can escape from OIS induced by H-RasV12. We designated these OIS-escaped cells as OISEC (OIS-escaped cells). OISEC lost the expression of p16 which plays an important role for cell cycle arrest for induction of senescence, but OISEC preserved the p16 expression machinery and exhibited senescence by the treatment with hydrogen peroxide (H2O2) as stress-induced premature senescence (SIPS). OISEC did not possess anchorage-independent growth potential, and functional disruption of p53 and Rb by SV40 early region encoding large T and small t antigens, induced the aneuploidy phenotype and colony-forming potential of OISEC together with the exhibition of in vivo tumor formation. Finally, we also found that the distinctive feature of OISEC is expression of transcription factors, Oct3/4, SOX2, and Nanog which is closely related to stem-like cell features. This study highlights the presence of a cell population which escaped from OIS, and this OISEC may transform into malignant cancer cells by the additional hits of several genes in vivo.
Type: article (author version)
URI: http://hdl.handle.net/2115/46956
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高阪 真路

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

 - Hokkaido University