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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
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8-Aminoadenosine Enhances Radiation-induced Cell Death in Human Lung Carcinoma A549 Cells
| Title: | 8-Aminoadenosine Enhances Radiation-induced Cell Death in Human Lung Carcinoma A549 Cells |
| Authors: | MEIKE, Shunsuke Browse this author | | YAMAMORI, Tohru Browse this author | | YASUI, Hironobu Browse this author | | EITAKI, Masato Browse this author | | MATSUDA, Akira Browse this author →KAKEN DB | | INANAMI, Osamu Browse this author →KAKEN DB |
| Keywords: | Radiosensitization | | 8-Aminoadenosine (8-NH2-Ado) | | Apoptosis |
| Issue Date: | 2011 |
| Journal Title: | Journal of Radiation Research |
| Volume: | 52 |
| Issue: | 4 |
| Start Page: | 456 |
| End Page: | 463 |
| Publisher DOI: | 10.1269/jrr.10188 |
| PMID: | 21785234 |
| Abstract: | The combination of a chemotherapeutic agent and radiation is widely applied to enhance cell death in solid tumor cells in cancer treatment. The purine analogue 8-aminoadenosine (8-NH2-Ado) is known to be a transcription inhibitor that has proved very effective in multiple myeloma cell lines and primary indolent leukemia cells. In this report, to examine whether 8-NH2-Ado had the ability to enhance the radiation-induced cell killing in solid tumor cells, human lung adenocarcinoma A549 cells were irradiated in the presence and absence of 8-NH2-Ado. 8-NH2-Ado significantly increased reproductive cell death and apoptosis in A549 cells exposed to X-rays. When peptide inhibitors against caspase-3, -8, and -9 were utilized to evaluate the involvement of caspases, all inhibitors suppressed the enhancement of radiation-induced apoptosis, suggesting that not only mitochondria-mediated apoptotic signal transduction pathways but also death receptor-mediated pathways were involved in this enhancement of apoptosis. In addition, in the cells exposed to the treatment combining X-irradiation and 8-NH2-Ado, reduction of the intracellular ATP concentration was essential for survival, and down-regulation of the expression of antiapoptotic proteins such as survivin and XIAP was observed. These results indicate that 8-NH2-Ado has potential not only as an anti-tumor drug for leukemia and lymphoma but also as a radiosensitizing agent for solid tumors. |
| Description: | JOI： JST.JSTAGE/jrr/10188 |
| Type: | article |
| URI: | http://hdl.handle.net/2115/46979 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 稲波 修
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