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Sonodynamic therapy using water-dispersed TiO2-polyethylene glycol compound on glioma cells : Comparison of cytotoxic mechanism with photodynamic therapy

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Title: Sonodynamic therapy using water-dispersed TiO2-polyethylene glycol compound on glioma cells : Comparison of cytotoxic mechanism with photodynamic therapy
Authors: Yamaguchi, Shigeru Browse this author
Kobayashi, Hiroyuki Browse this author
Narita, Takuhito Browse this author
Kanehira, Koki Browse this author
Sonezaki, Shuji Browse this author
Kudo, Nobuki Browse this author
Kubota, Yoshinobu Browse this author
Terasaka, Shunsuke Browse this author →KAKEN DB
Houkin, Kiyohiro Browse this author
Keywords: Glioma
Fluorescence assay
Photodynamic therapy
Polyethylene glycol
Sonodynamic therapy
Water-dispersed titanium dioxide
Issue Date: Sep-2011
Publisher: Elsevier B.V.
Journal Title: Ultrasonics Sonochemistry
Volume: 18
Issue: 5
Start Page: 1197
End Page: 1204
Publisher DOI: 10.1016/j.ultsonch.2010.12.017
PMID: 21257331
Abstract: Sonodynamic therapy is expected to be a novel therapeutic strategy for malignant gliomas. The titanium dioxide (TiO2) nanoparticle, a photosensitizer, can be activated by ultrasound. In this study, by using water-dispersed TiO2 nanoparticles, an in vitro comparison was made between the photodynamic and sonodynamic damages on U251 human glioblastoma cell lines. Water-dispersed TiO2 nanoparticles were constructed by the adsorption of chemically modified polyethylene glycole (PEG) on the TiO2 surface (TiO2/PEG). To evaluate cytotoxicity, U251 monolayer cells were incubated in culture medium including 100 μg/ml of TiO2/PEG for three hours and subsequently irradiated by ultraviolet light (5.0 mW/cm2) or 1.0 MHz ultrasound (1.0 W/cm2). Cell survival was estimated by MTT assay 24 hours after irradiation. In the presence of TiO2/PEG, the photodynamic cytotoxic effect was not observed after 20 minutes of an ultraviolet light exposure, while the sonodynamic cytotoxicity effect was almost proportional to the time of sonication. In addition, photodynamic cytotoxicity of TiO2/PEG was almost completely inhibited by radical scavenger, while suppression of the sonodynamic cytotoxic effect was not significant. Results of various fluorescent stains showed that ultrasound-treated cells lost their viability immediately after irradiation, and cell membranes were especially damaged in comparison with ultraviolet-treated cells. These findings showed a potential application of TiO2/PEG to sonodynamic therapy as a new treatment of malignant gliomas and suggested that the mechanism of TiO2/PEG mediated sonodynamic cytotoxicity differs from that of photodynamic cytotoxicity.
Type: article (author version)
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 寺坂 俊介

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