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Chromatography of carbon nanotubes separated albumin from other serum proteins : a method for direct analysis of their interactions

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Title: Chromatography of carbon nanotubes separated albumin from other serum proteins : a method for direct analysis of their interactions
Authors: Kuboki, Yoshinori Browse this author
Koshikawa, Takamitu Browse this author
Takita, Hiroko Browse this author →KAKEN DB
Fujisawa, Ryuichi Browse this author
Lee, Min-ho Browse this author
Abe, Shigeaki Browse this author
Akasaka, Tsukasa Browse this author
Uo, Motohiro Browse this author
Watari, Fumio Browse this author
Sammons, Rachel Browse this author
Keywords: Carbon nanotubes
Proteins
Chromatography
Issue Date: Jul-2010
Publisher: Japanese Society for Dental Materials and Devices
Journal Title: Dental Materials Journal
Volume: 29
Issue: 4
Start Page: 369
End Page: 373
Publisher DOI: 10.4012/dmj.2009-097
Abstract: Chromatography technology was employed to clarify the mechanism of interaction between multi-wall carbon nanotubes (MWCNT) and proteins. A column (16×100 mm) was packed with purified MWCNT, and various proteins were eluted with phosphate buffered saline (PBS) with and without gradient systems. It was found that albumin in bovine serum was eluted immediately from the column without any adsorption to MWCNT. Conversely, the non-albumin proteins, including a protein of 85 kDa molecular mass and a group of proteins with molecular masses higher than 115 kDa, exhibited considerably high affinity towards MWCNT. A sample of pure bovine serum albumin was also eluted immediately from the column, while lysozyme did not elute as a peak with PBS, but eluted with 0.6 M NaCl. Fundamentally, carbon nanotubes are devoid of any electrical charge. Therefore, other forces including the hydrogen bonds, hydrophilic interactions, and van der Waals forces were most probably responsible for the differential elution behaviors. In conclusion, this chromatographic method provided a simple and direct analysis of the interactions between carbon nanotubes and the various proteins.
Rights: Copyright © 2010 The Japanese Society for Dental Materials and Devices
Type: article
URI: http://hdl.handle.net/2115/47070
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 瀧田 裕子

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