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Screening and analysis of genes expressed upon infection of broad bean with Clover yellow vein virus causing lethal necrosis

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Title: Screening and analysis of genes expressed upon infection of broad bean with Clover yellow vein virus causing lethal necrosis
Authors: Nakahara, Kenji S. Browse this author →KAKEN DB
Kitazawa, Hiroaki Browse this author
Atsumi, Go Browse this author
Choi, Sun Hee Browse this author
Suzuki, Yuji Browse this author
Uyeda, Ichiro Browse this author →KAKEN DB
Issue Date: 18-Jul-2011
Publisher: BioMed Central
Journal Title: Virology Journal
Volume: 8
Start Page: 355
Publisher DOI: 10.1186/1743-422X-8-355
Abstract: Clover yellow vein virus (ClYVV) causes lethal systemic necrosis in legumes, including broad bean (Vicia faba) and pea (Pisum sativum). To identify host genes involved in necrotic symptom expression after ClYVV infection, we screened cDNA fragments in which expression was changed in advance of necrotic symptom expression in broad bean (V. faba cv. Wase) using the differential display technique and secondarily with Northern blot analysis. Expression changes were confirmed in 20 genes, and the six that exhibited the most change were analyzed further. These six genes included a gene that encodes a putative nitrate-induced NOI protein (VfNOI), and another was homologous to an Arabidopsis gene that encodes a glycine- and proline-rich protein GPRP (VfGPRP). We recently reported that necrotic symptom development in ClYVV-infected pea is associated with expression of salicylic acid (SA)-dependent pathogenesis-related (PR) proteins and requires SA-dependent host responses. Interestingly, VfNOI and VfGPRP expression was correlated with that of the putative SA-dependent PR proteins in ClYVV-infected broad bean. However, broad bean infected with a recombinant ClYVV expressing the VfGPRP protein showed weaker symptoms and less viral multiplication than that infected with ClYVV expressing the GFP protein. These results imply that VfGPRP plays a role in defense against ClYVV rather than in necrotic symptom expression.
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 中原 健二

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