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Prevalence and Carrier Status of Leptospirosis in Smallholder Dairy Cattle and Peridomestic Rodents in Kandy, Sri Lanka

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Title: Prevalence and Carrier Status of Leptospirosis in Smallholder Dairy Cattle and Peridomestic Rodents in Kandy, Sri Lanka
Authors: Gamage, Chandika D. Browse this author
Koizumi, Nobuo Browse this author
Muto, Maki Browse this author
Nwafor-Okoli, Chinyere Browse this author
Kurukurusuriya, Shanika Browse this author
Rajapakse, Jayanthe R. P. V. Browse this author
Kularatne, Senanayake A. M. Browse this author
Kanda, Koji Browse this author
Lee, Romeo B. Browse this author
Obayashi, Yoshihide Browse this author
Watanabe, Haruo Browse this author
Tamashiro, Hiko Browse this author →KAKEN DB
Keywords: Domestic Animals
Leptospirosis
PCR
Serology
Zoonosis
Issue Date: 8-Aug-2011
Publisher: Mary Ann Liebert
Journal Title: Vector-Borne and Zoonotic Diseases
Volume: 11
Issue: 8
Start Page: 1041
End Page: 1047
Publisher DOI: 10.1089/vbz.2010.0153
PMID: 21284522
Abstract: Leptospirosis is an important bacterial zoonotic disease globally and one of the notifiable diseases in Sri Lanka. Other than human leptospirosis, little information is available on leptospirosis in domestic and feral animals in Sri Lanka. Thus, this study attempted to determine the prevalence and carrier status of leptospirosis in small-holder dairy cattle and peridomestic rodents to understand the impact of the disease on public health in Kandy, Sri Lanka. Cattle and rodent samples were collected from the Yatinuwara and Udunuwara divisional secretaries in Kandy. Serum samples were analyzed for the presence of antileptospiral antibodies using microscopic agglutination test. DNA was extracted from cattle urine and rodent kidney tissue samples, in which polymerase chain reaction was carried out to detect the Leptospira flaB gene. The cattle in 19 (38.8%) of the 49 farms harbored antileptospiral antibodies. Out of 113 cattle serum samples, 23 (20.3%) were positive; 17 (73.9%) and 6 (26.1%) reacted with serogroups Sejroe and Hebdomadis, respectively. Out of the 74 rodent samples, 13 (17.5%) were positive; 8 (61.5%) and 4 (30.8%) had reactions to serogroups Javanica and Icterohaemorrhagiae, respectively. Leptospiral DNA was detected in one cattle urine sample and identified as Leptospira interrogans. This study revealed a high prevalence of leptospirosis in cattle and rodents in Kandy. These animals were infected with a wide array of leptospiral serogroups, which are consistent with the research findings observed in humans in Kandy. Overall, serological data indicate that relative to rodents, cattle may be a more significant reservoir for human transmission and a greater source of potential risk to local agricultural communities.
Rights: This is a copy of an article published in the Vector-Borne and Zoonotic Diseases © 2011 copyright Mary Ann Liebert, Inc.; Vector-Borne and Zoonotic Diseases is available online at: http://www.liebertonline.com.
Type: article
URI: http://hdl.handle.net/2115/47130
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 玉城 英彦

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