| Title: | Unique heparan sulfate from shrimp heads exhibits a strong inhibitory effect on infections by dengue virus and Japanese encephalitis virus |
| Authors: | Chen, Jiancheng Browse this author |
| Yamada, Shuhei Browse this author →KAKEN DB |
| Hama, Yoshiki Browse this author |
| Shetty, Ajaya Kumar Browse this author |
| Kobayashi, Takanari Browse this author |
| Oda, Hiroshi Browse this author |
| Seiki, Kosuke Browse this author |
| Kim, Eunmi Browse this author |
| Kimura, Takashi Browse this author |
| Takahashi, Naonori Browse this author |
| Hidari, Kazuya I. P. J. Browse this author |
| Suzuki, Takashi Browse this author |
| Suzuki, Yasuo Browse this author |
| Sugahara, Kazuyuki Browse this author |
| Keywords: | Glycosaminoglycans |
| Heparan sulfate |
| Dengue virus |
| Japanese encephalitis virus |
| Anticoagulant activity |
| Issue Date: | 19-Aug-2011 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 412 |
| Issue: | 1 |
| Start Page: | 136 |
| End Page: | 142 |
| Publisher DOI: | 10.1016/j.bbrc.2011.07.059 |
| PMID: | 21806963 |
| Abstract: | The structure and biological activities of a highly sulfated heparan sulfate (HS) extracted from shrimp (Penaeus brasiliensis) heads were characterized. Structurally the shrimp HS was more heterogenous than heparin, although it is still highly sulfated. The molecular mass of the shrimp HS preparation was determined to be 32.3 kDa by gel filtration HPLC. Analysis by surface plasmon resonance demonstrated that various growth/differentiation factors specifically bound to the shrimp HS with comparable affinity. Notably, the shrimp HS had a greater inhibitory effect against infections by dengue virus type 2 as well as Japanese encephalitis virus than heparin. Experiments on anticoagulant activity indicated that the shrimp HS exhibited significant anti-thrombin activity, but less than the commercial heparin. Hence, the HS preparation from shrimp heads, an industrial waste, is a prospective agent for a variety of clinical applications. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/47200 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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