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Unique heparan sulfate from shrimp heads exhibits a strong inhibitory effect on infections by dengue virus and Japanese encephalitis virus

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Title: Unique heparan sulfate from shrimp heads exhibits a strong inhibitory effect on infections by dengue virus and Japanese encephalitis virus
Authors: Chen, Jiancheng Browse this author
Yamada, Shuhei Browse this author
Hama, Yoshiki Browse this author
Shetty, Ajaya Kumar Browse this author
Kobayashi, Takanari Browse this author
Oda, Hiroshi Browse this author
Seiki, Kosuke Browse this author
Kim, Eunmi Browse this author
Kimura, Takashi Browse this author
Takahashi, Naonori Browse this author
Hidari, Kazuya I. P. J. Browse this author
Suzuki, Takashi Browse this author
Suzuki, Yasuo Browse this author
Sugahara, Kazuyuki Browse this author
Keywords: Glycosaminoglycans
Heparan sulfate
Dengue virus
Japanese encephalitis virus
Anticoagulant activity
Issue Date: 19-Aug-2011
Publisher: Elsevier
Journal Title: Biochemical and Biophysical Research Communications
Volume: 412
Issue: 1
Start Page: 136
End Page: 142
Publisher DOI: 10.1016/j.bbrc.2011.07.059
Abstract: The structure and biological activities of a highly sulfated heparan sulfate (HS) extracted from shrimp (Penaeus brasiliensis) heads were characterized. Structurally the shrimp HS was more heterogenous than heparin, although it is still highly sulfated. The molecular mass of the shrimp HS preparation was determined to be 32.3 kDa by gel filtration HPLC. Analysis by surface plasmon resonance demonstrated that various growth/differentiation factors specifically bound to the shrimp HS with comparable affinity. Notably, the shrimp HS had a greater inhibitory effect against infections by dengue virus type 2 as well as Japanese encephalitis virus than heparin. Experiments on anticoagulant activity indicated that the shrimp HS exhibited significant anti-thrombin activity, but less than the commercial heparin. Hence, the HS preparation from shrimp heads, an industrial waste, is a prospective agent for a variety of clinical applications.
Type: article (author version)
URI: http://hdl.handle.net/2115/47200
Appears in Collections:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山田 修平

 

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