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Babesia gibsoni : Detection in blood smears and formalin-fixed, paraffin-embedded tissues using deoxyribonucleic acid in situ hybridization analysis

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Title: Babesia gibsoni : Detection in blood smears and formalin-fixed, paraffin-embedded tissues using deoxyribonucleic acid in situ hybridization analysis
Authors: Yamasaki, Masahiro Browse this author →KAKEN DB
Kobayashi, Yusuke Browse this author
Nakamura, Kensuke Browse this author
Sasaki, Noboru Browse this author
Murakami, Masahiro Browse this author
Rajapakshage, Bandula Kumara Wickramasekara Browse this author
Ohta, Hiroshi Browse this author →KAKEN DB
Yamato, Osamu Browse this author
Maede, Yoshimitsu Browse this author →KAKEN DB
Takiguchi, Mitsuyoshi Browse this author →KAKEN DB
Keywords: Babesia gibsoni
In situ hybridization
Heat shock protein 70
Blood smear
Formalin-fixed
Paraffin-embedded tissues
Issue Date: Jan-2011
Publisher: Elsevier
Journal Title: Experimental Parasitology
Volume: 127
Issue: 1
Start Page: 119
End Page: 126
Publisher DOI: 10.1016/j.exppara.2010.07.004
PMID: 20637756
Abstract: In the present study, we attempted to detect Babesia gibsoni in blood smears and formalin-fixed, paraffin-embedded tissues obtained from B. gibsoni-infected dogs using in situ hybridization. Using a digoxigenin-conjugated deoxyribonucleic acid (DNA) probe, both intraerythrocytic and exoerythrocytic parasites in the culture could be specifically stained in blood smears fixed with 4% phosphate-buffered paraformaldehyde. This indicated that genomic DNA extracted from the parasites could be detected using in situ hybridization. Moreover, the parasite could be specifically stained in paraffin-embedded spleen, lymph node, and kidney sections using in situ hybridization. Infected erythrocytes in blood vessels in the spleen and kidney, hemosiderin-laden macrophages in the spleen, and phagocytized erythrocytes, which seemed to be infected with the parasites, in lymph nodes were also specifically stained. This suggests that in situ hybridization can be utilized to investigate both the life cycle of B. gibsoni and the pathological condition of canine babesiosis.
Type: article (author version)
URI: http://hdl.handle.net/2115/47276
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山崎 真大

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