| Title: | Babesia gibsoni : Detection in blood smears and formalin-fixed, paraffin-embedded tissues using deoxyribonucleic acid in situ hybridization analysis |
| Authors: | Yamasaki, Masahiro Browse this author →KAKEN DB |
| Kobayashi, Yusuke Browse this author |
| Nakamura, Kensuke Browse this author |
| Sasaki, Noboru Browse this author |
| Murakami, Masahiro Browse this author |
| Rajapakshage, Bandula Kumara Wickramasekara Browse this author |
| Ohta, Hiroshi Browse this author →KAKEN DB |
| Yamato, Osamu Browse this author |
| Maede, Yoshimitsu Browse this author →KAKEN DB |
| Takiguchi, Mitsuyoshi Browse this author →KAKEN DB |
| Keywords: | Babesia gibsoni |
| In situ hybridization |
| Heat shock protein 70 |
| Blood smear |
| Formalin-fixed |
| Paraffin-embedded tissues |
| Issue Date: | Jan-2011 |
| Publisher: | Elsevier |
| Journal Title: | Experimental Parasitology |
| Volume: | 127 |
| Issue: | 1 |
| Start Page: | 119 |
| End Page: | 126 |
| Publisher DOI: | 10.1016/j.exppara.2010.07.004 |
| PMID: | 20637756 |
| Abstract: | In the present study, we attempted to detect Babesia gibsoni in blood smears and formalin-fixed, paraffin-embedded tissues obtained from B. gibsoni-infected dogs using in situ hybridization. Using a digoxigenin-conjugated deoxyribonucleic acid (DNA) probe, both intraerythrocytic and exoerythrocytic parasites in the culture could be specifically stained in blood smears fixed with 4% phosphate-buffered paraformaldehyde. This indicated that genomic DNA extracted from the parasites could be detected using in situ hybridization. Moreover, the parasite could be specifically stained in paraffin-embedded spleen, lymph node, and kidney sections using in situ hybridization. Infected erythrocytes in blood vessels in the spleen and kidney, hemosiderin-laden macrophages in the spleen, and phagocytized erythrocytes, which seemed to be infected with the parasites, in lymph nodes were also specifically stained. This suggests that in situ hybridization can be utilized to investigate both the life cycle of B. gibsoni and the pathological condition of canine babesiosis. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/47276 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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