HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >

Detection of anti-Babesia gibsoni heat shock protein 70 antibody and anti-canine heat shock protein 70 antibody in sera from Babesia gibsoni-infected dogs

Files in This Item:
VP180-3-4_215-225.pdf4.5 MBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/47277

Title: Detection of anti-Babesia gibsoni heat shock protein 70 antibody and anti-canine heat shock protein 70 antibody in sera from Babesia gibsoni-infected dogs
Authors: Yamasaki, Masahiro Browse this author →KAKEN DB
Ishida, Mikiko Browse this author
Nakamura, Kensuke Browse this author
Sasaki, Noboru Browse this author
Murakami, Masahiro Browse this author
Kumara, Wickramasekara Rajapakshage Bandula Browse this author
Tamura, Yu Browse this author
Lim, Sue Yee Browse this author
Ohta, Hiroshi Browse this author →KAKEN DB
Takiguchi, Mitsuyoshi Browse this author →KAKEN DB
Keywords: Babesia gibsoni
Heat shock protein 70
Anti-heat shock protein 70 antibody
Issue Date: 25-Aug-2011
Publisher: Elsevier B.V.
Journal Title: Veterinary Parasitology
Volume: 180
Issue: 3-4
Start Page: 215
End Page: 225
Publisher DOI: 10.1016/j.vetpar.2011.03.006
PMID: 21454016
Abstract: Antibodies that recognized either Babesia gibsoni or canine red blood cell (RBC) 70-kilodalton (kDa) protein were detected in serum from acutely and chronically B. gibsoni-infected. In those sera, antibodies that reacted with recombinant B. gibsoni and canine heat shock protein 70 (rBgHsp70 and rcHsp70) were detected; therefore, B. gibsoni and canine RBC 70-kDa proteins seemed to be BgHsp70 and cHsp70, respectively. In infected dogs, the amounts of these antibodies increased after infection. Interestingly, polyclonal antibody raised against rBgHsp70 in two rabbits reacted not only with rBgHsp70 but also with rcHsp70 and native cHsp70 from canine RBCs. Because BgHsp70 showed high homology with cHsp70 (70.8%), anti-rBgHsp70 antibody might cross-react with cHsp70. Additionally, the localizations of both BgHsp70 and cHsp70 were observed by indirect fluorescence assay. As a result, cHsp70 was not found on the membrane surface of erythrocytes, suggesting that erythrocytes would not be targets of anti-cHsp70 antibody. Meanwhile, only exoerythrocytic parasites were stained by anti-rBgHsp70 antibody. This result showed that BgHsp70 would be expressed on the surface of parasites during the exoerythrocytic stage. These results indicated that BgHsp70 was a highly immunogenic protein in canine B. gibsoni infection, and that exoerythrocytic parasites might be targets of anti-BgHsp70 antibody.
Type: article (author version)
URI: http://hdl.handle.net/2115/47277
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山崎 真大

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

Feedback - Hokkaido University