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Identification of antigenic peptides derived from B-cell epitopes of nucleocapsid protein of mouse hepatitis virus for serological diagnosis

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/47279

Title: Identification of antigenic peptides derived from B-cell epitopes of nucleocapsid protein of mouse hepatitis virus for serological diagnosis
Authors: Asano, Atsushi Browse this author
Torigoe, Daisuke Browse this author
Sasaki, Nobuya Browse this author →KAKEN DB
Agui, Takashi Browse this author →KAKEN DB
Keywords: Diagnosis
ELISA
Epitope mapping
Mouse hepatitis virus
Nucleocapsid protein
Issue Date: Oct-2011
Publisher: Elsevier B.V.
Journal Title: Journal of Virological Methods
Volume: 177
Issue: 1
Start Page: 107
End Page: 111
Publisher DOI: 10.1016/j.jviromet.2011.07.006
Abstract: Mouse hepatitis virus (MHV) infection is found commonly in laboratory mice and this virus has been known to cause various diseases such as subclinical infection, enteritis, hepatitis, and encephalitis. Serological tests are used commonly to diagnose MHV infection. Complete MHV virions have been used primarily as antigens for serological diagnosis to date. To develop an antigen that is more specific, more sensitive, and easier to prepare for serological diagnosis, the antigenic sites in the MHV-nucleocapsid (N) protein were screened in this study. Sixteen antigenic linear sequences in the N protein were found using antisera obtained from mice infected naturally with MHV and a peptide array containing overlapping 10-mer peptides covering the entire N protein. From these antigenic sequences, two synthesized peptides, ILKKTTWADQTERGL and RFDSTLPGFETIMKVL, which were consistent with positions 24-38 and 357-372 of the N protein respectively, were used as antigens in ELISA. Evaluation of ELISA with these peptides revealed that both peptides were specific to anti-MHV antisera. Furthermore, ELISA performed using these peptides was more sensitive than commercial ELISA used for a screening sera from mice infected accidentally to MHV maintained in cages, suggesting that these peptides are useful for serological diagnosis of MHV infection.
Type: article (author version)
URI: http://hdl.handle.net/2115/47279
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 安居院 高志

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