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Leishmania species identification using FTA card sampling directly from patients' cutaneous lesions in the state of Lara, Venezuela

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Title: Leishmania species identification using FTA card sampling directly from patients' cutaneous lesions in the state of Lara, Venezuela
Authors: Kato, Hirotomo Browse this author →KAKEN DB
Watanabe, Junko Browse this author
Mendoza Nieto, Iraida Browse this author
Korenaga, Masataka Browse this author
Hashiguchi, Yoshihisa Browse this author →KAKEN DB
Keywords: Venezuela
Leishmania venezuelensis
Leishmania mexicana
Leishmania braziliensis
Cytochrome b
rRNA internal transcribed spacer
Issue Date: Oct-2011
Publisher: Elsevier
Journal Title: Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume: 105
Issue: 10
Start Page: 561
End Page: 567
Publisher DOI: 10.1016/j.trstmh.2011.05.009
PMID: 21907375
Abstract: A molecular epidemiological study was performed using FTA card materials directly sampled from lesions of patients with cutaneous leishmaniasis (CL) in the state of Lara, Venezuela, where causative agents have been identified as Leishmania (Viannia) braziliensis and L. (Leishmania) venezuelensis in the past studies. Of the seventeen patients diagnosed with CL, Leishmania species were successfully identified in sixteen patients based on analysis of the cytochrome b and ribosomal RNA internal transcribed spacer sequences. Consistent with previous findings, seven of the patients were infected with L. (V.) braziliensis. However, parasites from the other nine patients were genetically identified as L. (L.) mexicana, which differed from results of previous enzymatic and antigenic analyses. These results strongly suggest that L. (L.) venezuelensis is a variant of L. (L.) mexicana, and the classification of L. (L.) venezuelensis should be reconsidered.
Type: article (author version)
URI: http://hdl.handle.net/2115/47383
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 加藤 大智

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