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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
Peer-reviewed Journal Articles, etc >
Design of Mesoporous Silica/Cytosine−Phosphodiester−Guanine Oligodeoxynucleotide Complexes To Enhance Delivery Efficiency
| Title: | Design of Mesoporous Silica/Cytosine−Phosphodiester−Guanine Oligodeoxynucleotide Complexes To Enhance Delivery Efficiency |
| Authors: | Zhu, Yufang Browse this author | | Meng, Wenjun Browse this author | | Li, Xianglan Browse this author | | Gao, Hong Browse this author | | Hanagata, Nobutaka Browse this author |
| Issue Date: | 2011 |
| Publisher: | American Chemical Society |
| Journal Title: | The Journal of Physical Chemistry C |
| Volume: | 115 |
| Issue: | 2 |
| Start Page: | 447 |
| End Page: | 452 |
| Publisher DOI: | 10.1021/jp109535d |
| Abstract: | We developed a potential cytosine-phosphodiester-guanine oligodeoxynucleotide (CpG ODN) delivery system combining amino-modified mesoporous silica SBA-15 (SBA-NH2) particles with polycation poly(allylamine hydrochloride) (PAH) to form SBA-ODN-PAH complexes. Stability, cell uptake, in vitro cytotoxicity, and the nuclear factor κB (NF-κB) activity of the SBA-ODN-PAH complexes were evaluated. Gel electrophoresis indicated that the SBA-ODN-PAH complexes exhibited enhanced serum stability due to the dual protection effects of the SBA-NH2 particles and the PAH coating. The SBA-ODN-PAH complexes were taken up by 293XL-hTLR9 cells and little cytotoxicity was expressed in a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay. Most importantly, the SBA-ODN-PAH complexes significantly enhanced NF-κB activity, stimulated by interaction between the internalized CpG ODN and Toll-like receptor 9, compared to free CpG ODN and SBA-ODN complexes. Thus, SBA-ODN-PAH complexes provide a promising strategy for enhancing the delivery efficiency of CpG ODN. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/47436 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 花方 信孝
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